Mycobacterium peregrinum Kusunoki and Ezaki
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2. From a single tube of #1395 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette and use to rehydrate the entire pellet.
3. Aseptically transfer the rehydrated pellet back into the broth tube. Mix well.
4. Heavily inoculate a slant of #90 and/or a plate of #173 agar.
5. After growth has been achieved, additional transfers may be made.
Growth on solid medium requires between three to seven days. Colonies on #173 plates are smooth, entire, circular, high convex, opaque, and buff. With age, colonies appear filiform and irregular. Culture will also grow on Brain Heart Infusion Agar (BD 241830).
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J. Bacteriol. 81: 338-345, 1961.
Bojalil LF, et al. Adansonian classification of mycobacteria. J. Gen. Microbiol. 28: 333-346, 1962. PubMed: 13870716
Tsang AY, et al. Antigenic relationships of the Mycobacterium fortuitum-Mycobacterium chelonae complex. Int. J. Syst. Bacteriol. 34: 35-44, 1984.
Kusunoki S, Ezaki T. Proposal of Mycobacterium peregrinum sp. nov., nom. rev., and elevation of Mycobacterium chelonae subsp. abscessus (Kubica et al.) to species status: Mycobacterium abscessus comb. nov. Int J Syst Bacteriol 42: 240-245, 1992. PubMed: 1581184