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Vibrio harveyi (Johnson and Shunk) Baumann et al.


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Type strain. Produces restriction endonuclease VhaI.
Product category
Strain designation
[NCMB 1280]
Type strain
Genome sequenced strain
Isolation source
Dead luminescing amphipod, Talorchestia sp.
Geographical isolation
United States; Massachusetts; Woods Hole
Product format
Storage conditions
2°C to 8°C
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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information


Specific applications
Produces restriction endonuclease VhaI

Handling information

Handling procedure
  1. Open vial according to enclosed instructions.
  2. Using a single tube of #101 broth (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a Pasteur or 1.0 mL pipette. Rehydrate the entire pellet.
  3. Aseptically transfer this aliquot back into the broth tube. Mix well.
  4. Use several drops of the suspension to inoculate a #101 agar slant and/or plate.
  5. Incubate the tubes and plate at 26°C for 24 to 48 hours.
Handling notes

This strain will grow in #2 Marine Broth 2216 (BD 279110) and on #2 Marine Agar 2216 (BD 212185), but Photobacterium Medium is required for demonstrating luminescence.

To check for luminescence, inoculate a #101 slant, leaving cap loose. Incubate at optimal temperature until growth is observed. Then, hold in the dark for 10 minutes. If no luminescence is detected, reincubate and check again after 24 to 48 additional hours.

When maximum luminescence is obtained, close stopper tightly. Demonstration of luminescence requires that vial be rehydrated with #101 broth. Transfers from #101 broth to #2 broth may yield more growth.

Colonies on #101 agar are circular, entire, glistening, and smooth.

Additional information on this culture is available on the ATCC® web site at

Quality control specifications

Verification method
Whole-genome Sequencing


Deposited as
Photobacterium harveyi (Johnson and Shunk) Breed and Lessel
FH Johnson
Type of isolate
Cross references
GenBank D11205 Vibiro harveyi 16S rRNA.
GenBank X56578 V.harveyi 16S ribosomal RNA.
GenBank X74706 V.harveyi (ATCC 14126T) gene for 16S ribosomal RNA.
GenBank AB047588 Vibrio harveyi rnpB, ORF1, ORF2 genes, partial cds.
GenBank M29762 V.harveyi Arg-tRNA, two Pro-tRNA and two His-tRNA genes.
GenBank D14674 Vibrio harveyi gene for NADH:FMN oxidoreductase, complete cds.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.


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While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

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Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.


Frequently Asked Questions


Curated Citations

Baumann P, et al. Reevaluation of the taxonomy of Vibrio, Beneckea, and Photobacterium: abolition of the genus Beneckea. Curr. Microbiol. 4: 127-132, 1980.

Hendrie MS, et al. The identification, taxonomy and classification of luminous bacteria. J. Gen. Microbiol. 64: 151-169, 1970.

Int. Bull. Bacteriol. Nomencl. Taxon. 12: 77, 1962.

Johnson FH, Shunk IV. An interesting new species of luminous bacteria. J. Bacteriol. 31: 585-592, 1936.

Roberts RJ. Restriction and modification enzymes and their recognition sequences. Nucleic Acids Res. 13: r165-r200, 1985. PubMed: 2987885

View All Curated Citations for this Product

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