Corynebacterium herculis Dunn et al.
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2. Using a single tube of #3 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #3 slant, and/or plate
5. Incubate all tubes and plates at 37oC for 24 hours.
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Dunn CG, et al. Production of L-glutamic acid and alpha-ketoglutaric acids. US Patent 3,120,472 dated Feb 4 1964
Katsumata R, et al. Plasmids. US Patent 4,500,640 dated Feb 19 1985