Rhodococcus rhodochrous (Zopf) Tsukamura emend. Rainey et al.
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2. From a single tube of #44 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette and use to rehydrate the pellet.
3. Aseptically transfer the rehydrated pellet back into the broth tube. Mix well.
4. Use several drops of this suspension to inoculate an additional broth tube, a #44 agar slant and/or a plate.
5. Incubate the tubes and plate at 26°C for three to five days.
Cells are pleomorphic coccobacilli.
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Gordon RE, Mihm JM. A comparison of four species of mycobacteria. J. Gen. Microbiol. 21: 736-748, 1959. PubMed: 13851229
Goodfellow M, Alderson G. The actinomycetes-genus Rhodococcus: a home for the "rhodochrous" complex. J. Gen. Microbiol. 100: 99-122, 1977. PubMed: 874450
Rainey FA, et al. Polyphasic evidence for the transfer of Rhodococcus roseus to Rhodococcus rhodochrous. Int. J. Syst. Bacteriol. 45: 101-103, 1995.
Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.