Zimmermannella faecalis Lin et al.
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2. From a single tube of #44 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette and use to rehydrate the entire pellet.
3. Aseptically transfer the rehydrated pellet back into the broth tube. Mix well.
4. Use several drops of this suspension to inoculate an additional broth tube, a #44 agar slant and/or a plate.
5. Incubate the tubes and plate at 37°C for 24 to 48 hours.
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. . Proc. Soc. Exp. Biol. Med. 74: 571-573, 1950.
Lin YC, et al. Zimmermannella helvola gen. nov., sp. nov., Zimmermannella alba sp. nov., Zimmermannella bifida sp. nov., Zimmermannella faecalis sp. nov. and Leucobacter albus sp. nov., novel members of the family Microbacteriaceae. Int. J. Syst. Evol. Microbiol. 54: 1669-1676, 2004. PubMed: 15388726