Pseudomonas citronellolis Seubert
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2. Using a single tube of #3 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate an additional broth tube, a #3 agar slant and/or a plate.
5. Incubate all tubes and plate at 26°C for 24 hours.
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Kuzma J, et al. Bacteria produce the volatile hydrocarbon isoprene. Curr. Microbiol. 30: 97-103, 1995. PubMed: 7765889
Seubert W. Degradation of isoprenoid compounds by micro-organisms. I. Isolation and characterization of an isoprenoid-degrading bacterium, Pseudomonas citronellolis n. sp.. J. Bacteriol. 79: 426-434, 1960. PubMed: 14445211
Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.