Corynebacterium glutamicum (Kinoshita et al.) Abe et al.
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2. Using a single tube of #3 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #3 slant, and/or plate
5. Incubate all tubes and plates at 30oC for 24 hours.
Colonies on #3 agar are entire, glistening, smooth, convex, translucent, and grayish in color. Cells are short, non-motile rods occurring singly, in pairs, in V-formations, stacked or in small clusters.
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Motozaki M, et al. Process for producing L-glutamic acid by bacterial fermentation. Canadian Patent 634,377
Motozaki S, et al. Process for producing L-glutamic acid by bacterial fermentation. US Patent 3,128,237 dated Apr 7 1964
Okumura S, et al. Process for producing L-glutamic acid. US Patent 3,136,702 dated Jun 9 1964
Katsumata R, et al. Plasmids. US Patent 4,500,640 dated Feb 19 1985