Paracoccus denitrificans (Beijerinck and Minkman) Davis emend. Rainey et al.


Product category
Strain designation
(NRC) 449 [CCM 982, E-IV 3.1.1 (L450), NRRL B-3784]
Type strain
Isolation source
Product format
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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information


Specific applications
Produces blue copper protein amicyanin


Atcc strains 19367, 13543, and 17741 may represent the same original isolate

Handling information

Handling procedure
1. Open vial according to enclosed instructions.

2. From a single tube of #3 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette and use to rehydrate the entire pellet.           

3. Aseptically transfer the rehydrated pellet back into the broth tube.  Mix well.   

4. Use several drops of this suspension to inoculate an additional broth tube, a #3 agar slant and/or a plate.     

5. Incubate the tubes and plate at 26°C for 48 hours.

Handling notes
Colonies are circular, smooth, entire, convex and cream in color.  Strain is a Gram negative coccus and cells appear in pairs, singles and in tetrads.  The ability of the organism to grow autotrophically in the presence of hydrogen was tested and was found to be positive.

          Additional information on this culture is available on the ATCC web site at


Deposited as
Micrococcus denitrificans Beijerinck
MT Clement
Chain of custody
ATCC <-- MT Clement <-- A. Kluyver E-IV 3.1.1 (L450)
Type of isolate
Cross references
GenBank M17522 P.denitrificans bc1 operon genes encoding three subunits (FeS, b and c1) of the cytochrome bc1 complex, complete cds.
GenBank U31902 Paracoccus denitrificans succinate dehydrogenase b-type cytochrome subunit (sdhC), succinate dehydrogenase hydrophobic membrane anchor (sdhD), succinate dehydrogenase flavoprotein subunit (sdhA), and succinate dehydrogenase iron-sulfur protein sub

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If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.



Curated Citations

Husain M, Davidson VL. An inducible periplasmic blue copper protein from Paracoccus denitrificans. Purification, properties, and physiological role. J. Biol. Chem. 260: 14626-14629, 1985. PubMed: 2997215

Sharma KD, et al. Resonance Raman spectroscopy of amicyanin, a blue copper protein from Paracoccus denitrificans. J. Biol. Chem. 263: 3303-3306, 1988. PubMed: 2830281

Davis DH, et al. Proposal to reject the genus Hydrogenomonas: taxonomic implications. Int. J. Syst. Bacteriol. 19: 375-390, 1969.

Rainey FA, et al. A re-evaluation of the taxonomy of Paracoccus denitrificans and a proposal for the combination Paracoccus pantotrophus comb. nov.. Int. J. Syst. Bacteriol. 49: 645-651, 1999. PubMed: 10319488

Davis DH, et al. Taxonomic studies on some gram negative polarly flagellated "hydrogen bacteria" and related species. Arch. Mikrobiol. 70: 1-13, 1970. PubMed: 4987616

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