Paenibacillus larvae (White) Ash et al.
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2. Using a single tube of #3 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate an additional broth tube, a #3 agar slant and/or a plate.
5. Incubate all tubes and plate at 30°C for 48 to 72 hours.
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Katznelson H. Bacillus pulvifaciens, n. sp., an organism associated with powdery scale of honeybee larvae. J. Bacteriol. 59: 153-155, 1950.
Cody RM. Distribution of chitinase and chitobiase in Bacillus. Curr. Microbiol. 19: 201-205, 1989.