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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
2. From a single tube of the recommended broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette and use to rehydrate the entire pellet.
3. Aseptically transfer the rehydrated pellet back into the broth tube. Mix well.
4. Heavily inoculate a slant and/or a plate of #93 agar.
5. After growth has been achieved, additional transfers may be made.
Additional information on this culture is available on the ATCC web site at www.atcc.org.
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Davis JB, et al. Mycobacterium paraffinicum n. sp., a bacterium isolated from soil. Appl. Microbiol. 4: 310-315, 1956.
Wayne LG, et al. Semantide- and chemotaxonomy-based analyses of some problematic phenotypic clusters of slowly growing mycobacteria, a cooperative study of the international working group on mycobacterial taxonomy. Int. J. Syst. Bacteriol. 46: 280-297, 1996. PubMed: 8573508