Novispirillum itersonii subsp. itersonii (Giesberger) Yoon et al.
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2. Using a single tube of #3 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Incubate at 26oC for 48 hours with shaking.
5. After growth has been achieved, use several drops of the suspension to inoculate a second tube of broth, a slant, and/or plate.
This strain displays two colony types after 48 hours incubation: a) 75% circular, smooth, entire, convex, glistening, translucent; and b) 25% irregular, flat, rough, and transparent. Colony type b is a variant that has lost the ability to form helical cells (see Bergeys Manual of Systematic Bacteriology, Vol. 1, 1984, pg. 73). Biochemical characterization and the fatty acid profile are identical for both types.
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Hylemon PB, et al. The genus Spirillum: a taxonomic study. Int. J. Syst. Bacteriol. 23: 340-380, 1973.
Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.