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Staphylococcus epidermidis (Winslow and Winslow) Evans

12228-MINI-PACK

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ATCC® 12228-MINI-PACK™ consists of 6 ready-to-use vials of ATCC® 12228™ frozen in 200 µL of glycerol stock, eliminating the need to rehydrate and culture the strain prior to use. Each vial is provided with a 2-D barcode for easy storage and tracking, as well as peel-off labels for fast and reliable recordkeeping.
Product category
Bacteria
Strain designation
FDA strain PCI 1200
Type strain
No
Genome sequenced strain
Yes
Applications
Bioinformatics
Food testing
Media testing
Quality control
Product format
Frozen
Shipping information
6 ready-to-use vials containing the strain in glycerol stock
Storage conditions
-80°C or colder

Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

General

Specific applications
ATCC 12228-MINI-PACK consists of 6 ready-to-use vials of ATCC 12228 frozen in 200 µL of glycerol stock, eliminating the need to rehydrate and culture the strain prior to use. Each vial is provided with a 2-D barcode for easy storage and tracking, as well as peel-off labels for fast and reliable recordkeeping. Developed by the leaders in microbial cultivation and preservation, ATCC® Minis provide a convenient, ready-to-use solution for handling quality control strains. ATCC® Minis are authenticated and backed by ATCC polyphasic testing – ensuring the same consistent and reliable reference materials you’ve come to trust for ATCC Genuine Cultures. It is easy to ensure the quality of your products with ATCC® Minis – just open, plate, and go! .

Food testing
Pharmaceutical and Personal Care
Assay of gentamicin RefEuropean Pharmacopoeia Commission Microbiological assay of antibiotics. Strasbourg, France:European Pharmacopoeia Commission;European Pharmacopoeia EP 2.7.2, 1997 RefU.S. Pharmacopeia General Chapters; <81> Antibiotics-Microbial Assays. Rockville, MD: U.S. Pharmacopeia; USP USP34-NF29, 2011
Assay of neomycin RefU.S. Pharmacopeia General Chapters; <81> Antibiotics-Microbial Assays. Rockville, MD: U.S. Pharmacopeia; USP USP34-NF29, 2011 RefAOAC International Antibiotics in feeds, microbiological methods. Gaithersburg, MD:AOAC International;AOAC "Official Methods of Analysis of the AOAC International" 957.23. Ref Neomycin in Feeds -- Stahl Microbiological Agar Diffusion Assay. Gaithersburg, MD:AOAC International;AOAC "Official Methods of Analysis of the AOAC International" 998.02.
Assay of netilmycin RefU.S. Pharmacopeia General Chapters; <81> Antibiotics-Microbial Assays. Rockville, MD: U.S. Pharmacopeia; USP USP34-NF29, 2011
Assay of novobiocin RefU.S. Pharmacopeia General Chapters; <81> Antibiotics-Microbial Assays. Rockville, MD: U.S. Pharmacopeia; USP USP34-NF29, 2011RefAOAC International Antibiotics in feeds, microbiological methods. Gaithersburg, MD:AOAC International;AOAC "Official Methods of Analysis of the AOAC International" 957.23.
Assay of oleandomycin
Assay of paromomycin aminosidine, catenulin RefU.S. Pharmacopeia General Chapters; <81> Antibiotics-Microbial Assays. Rockville, MD: U.S. Pharmacopeia; USP USP34-NF29, 2011
Assay of sisomicin RefU.S. Pharmacopeia General Chapters; <81> Antibiotics-Microbial Assays. Rockville, MD: U.S. Pharmacopeia; USP USP34-NF29, 2011
Inhibition testing Ref Standard Test Method for Evaluation of Antimicrobial Formulations by the Agar Patch Technique. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 1882-05.
Media testing Ref Quality Control for Commercially Prepared Microbiological Culture Media; Approved Standard - 3rd Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M22-A3. Ref Microbiology of food and animal feeding stuffs--Guidelines on preparation and production of culture media-- Part 2: Practical guidelines on performance testing of culture media.. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 11133-2:2003. Ref Microbiology of food and animal feeding stuffs --- Guidelines on preparation and production of culture media --- Part 2: Practical guidelines on performance testing of culture media - Annex B: Recommended test microorganisms for commonly used culture media. London, UK:British Standards Institution;British Standard DD CEN ISO/TS 11133:2003.
Quality control strain Ref BD Crystal Gram-Positive QC Set. BD Becton Dickinson. 
Sterility testing
Susceptibility disc testing neomycin
Susceptibility disc testing vancomycin RefZhang YQ, et al. Genome-based analysis of virulence genes in a non-biofilm-forming Staphylococcus epidermidis strain (ATCC 12228). Mol. Microbiol. 49: 1577-1593, 2003. PubMed: 12950922
Preceptrol
No

Characteristics

Comments
Genome sequenced strain
Strain does not form a biofilm RefZhang YQ, et al. Genome-based analysis of virulence genes in a non-biofilm-forming Staphylococcus epidermidis strain (ATCC 12228). Mol. Microbiol. 49: 1577-1593, 2003. PubMed: 12950922
Sensitive to vancomycin RefZhang YQ, et al. Genome-based analysis of virulence genes in a non-biofilm-forming Staphylococcus epidermidis strain (ATCC 12228). Mol. Microbiol. 49: 1577-1593, 2003. PubMed: 12950922RefZhang YQ, et al. Genome-based analysis of virulence genes in a non-biofilm-forming Staphylococcus epidermidis strain (ATCC 12228). Mol. Microbiol. 49: 1577-1593, 2003. PubMed: 12950922

Handling information

Medium
Temperature
37°C
Atmosphere
Aerobic
Handling procedure
Frozen mini-cryovials packed in dry ice should either be thawed immediately for use or stored at or below
-70°C until the expiration date printed on the label. Storage at -20°C is acceptable for up to 1 year.
  1. Thaw the bacterial strain upright using gentle agitation in a 25°C to 30°C water bath. Thawing will be rapid; approximately 2-3 minutes or until all ice crystals have melted.
  2. Immediately after thawing, wipe down the ampoule with 70% ethanol and aseptically transfer the entire contents to a 5-6 mL tube of #3 broth or directly inoculate a #3 plate and/or #3 agar slant.
  3. Discard the empty vial. Do not refreeze any unused portion as it will result in a loss of viability.
  4. Incubate at 37°C for 24 hours.
Handling notes
Purified genomic DNA of this strain is available as ATCC 12228D-5.
This strain is also available as certified reference material ATCC CRM-12228.
Additional information on this culture is available on the ATCC web site at www.atcc.org.

Quality control specifications

Verification method
Whole-genome Sequencing

History

Deposited as
Staphylococcus epidermidis (Winslow and Winslow) Evans
Depositors
FDA
Special collection
ATCC® Minis
Cross references
GenBank U39771 Staphylococcus epidermidis 16S-23S ribosomal RNA spacer region.
GenBank U90018 Staphylococcus epidermidis 16S-23S ribosomal RNA spacer region.
GenBank U90019 Staphylococcus epidermidis 16S-23S ribosomal RNA spacer region.
GenBank U90020 Staphylococcus epidermidis 16S-23S ribosomal RNA spacer region.
GenBank U90021 Staphylococcus epidermidis 16S-23S ribosomal RNA spacer region.
GenBank AE015929 Staphylococcus epidermidis ATCC 12228, complete genome.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Frequently Asked Questions

References

Curated Citations

British Pharmacopoeia Commission Biological assay of antibiotics. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XIV A, 2003

European Pharmacopoeia Commission Microbiological assay of antibiotics. Strasbourg, France:European Pharmacopoeia Commission;European Pharmacopoeia EP 2.7.2, 1997

Quality Control for Commercially Prepared Microbiological Culture Media; Approved Standard - 3rd Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M22-A3.

U.S. Pharmacopeia General Chapters; <81> Antibiotics-Microbial Assays. Rockville, MD: U.S. Pharmacopeia; USP USP34-NF29, 2011

GEN III MicroPlate™ QC/ OmniLog® & MicroStation™ Validation Set

View All Curated Citations for this Product

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