Acidovorax facilis (Schatz and Bovell) Willems et al.
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2. Using a single tube of #72 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate an additional broth tube, a #72 agar slant and/or a plate.
5. Incubate all tubes and plate at 30°C for 48 hours.
This strain will also grow on Nutrient Agar (BD 213000) and Tryptic Soy Agar (BD 236950).
Three colony types are observed: a) round, entire, pulvinate, dark; b) smaller, lighter, flatter; and c) crenated, entire, and dark.
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Schatz A, Bovell C Jr.. Growth and hydrogenase activity of a new bacterium, Hydrogenomonas facilis. J. Bacteriol. 63: 87-98, 1952. PubMed: 14927551
Davis DH, et al. Proposal to reject the genus Hydrogenomonas: taxonomic implications. Int. J. Syst. Bacteriol. 19: 375-390, 1969.
Willems A, et al. Acidovorax, a new genus for Pseudomonas facilis, Pseudomonas delafieldii, E. Falsen (EF) group 13, EF group 16, and several clinical isolates, with the species Acidovorax facilis comb. nov., Acidovorax delafieldii comb. nov., and Acidovorax temperanssp. nov. Int. J. Syst. Bacteriol. 40: 384-398, 1990. PubMed: 2275854
Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.