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Organoid Culture Fundamentals: Critical Steps for Success

March 25, 2021, at 12:00 PM ET


The currently available preclinical cancer models are often inadequate for studying cancer biology, developing personalized therapeutics, and identifying unique biomarkers. To address this deficit, ATCC has collaborated with the Human Cancer Model Initiative (HCMI) to launch almost 150 patient-derived models, featuring over 80 organoids. The clinical and sequencing data for these advanced models are available through a linked portal to the HCMI searchable catalog and the Genomic Data Commons (GDC) data portal. In addition to providing the models, ATCC supports scientists who are new to culturing these complex microtissues by offering organoid culture protocols and complete medium formulations. In this webinar we present step-by-step instructions that thoroughly demonstrate the process of thawing, expanding, and cryopreserving organoids.

Key Points

  • Clinical and sequencing data for the HCMI models are available through a linked portal to the HCMI searchable catalog and the GDC data portal.
  • While 3-D organoid culture may seem intimidating, careful preparation and good work instructions, such as those contained in this webinar, will start you on the path to successfully culturing this advanced type of in vitro model.
  • ATCC further supports the HCMI models by offering organoid "CoreKits" that contain the necessary reagents for organoid expansion.

View the Presentation


Steve Budd, headshot.

Steven Budd, MS, MBA

Product Specialist, ATCC

Steven Budd is a Product Specialist that manages the cell culture reagents at ATCC. He has 6 years of experience in the product management of scientific tools. Before that, he gained 4 years of experience in biomedical research and cell culture as a research specialist at the University of North Carolina at Chapel Hill. Mr. Budd has a M.S. in Biology from the University of North Carolina at Wilmington and an M.B.A. from North Carolina State University.

Organoid resources

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Discover next-generation organoid models

During the drug discovery process, having access to models that recapitulate tumor properties is essential for predictive screening. To meet this need, ATCC has made it a priority to provide patient-derived 3-D organoid models annotated with molecular and clinical data that represent a variety of healthy and cancerous tissues. These next-generation cancer models are capable of long-term expansion in culture while remaining phenotypically and genetically stable. Further, they provide invaluable preclinical models for studying cancer and offer many advantages over existing human or non-human animal cancer models.

Explore patient-derived organoids
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Organoids Education

Culturing organoids can be intimidating. To help researchers get their organoids growing, ATCC scientists  created educational materials that contains everything you need to know about the initiation, expansion, and cryopreservation of organoids in embedded 3-D culture. Watch our instructional video on demand, or download the comprehensive organoid culture guide.

Learn More