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Fingers holding a test tube containing a silver, red, and blue DNA double helix.

Precise Counting of Targeted Nucleic Acids has Never Been Easier

Oct 30, 2014 at 12:00 PM ET

Abstract

Droplet Digital PCR (ddPCR) is an elegant technology that permits accurate and absolute quantification of target nucleic acid molecules. By subpartitioning a PCR reaction into thousands of nanoliter sized droplets, amplifying, and subsequently individually interrogating them, ddPCR allows for increased quantitative resolution, enhanced detection of difficult to amplify targets, better reproducibility, and simplified data analysis. This webinar will provide a technical overview of ddPCR from technical and workflow related perspectives as well as review key applications such as copy number variation (CNV) analysis, rare mutation detection (RMD) analysis, and standard free (stand-alone) absolute quantification of nucleic acids.

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Presenter

Francisco Bizouarn, headshot.

Francisco Bizouarn

Global Digital Applications Specialist, Digital Biology Center, Bio-Rad Laboratories