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Evaluation of ATCC Site-Specific Microbiome Standards on Long-Read Sequencing Platforms

Poster
DNA rods with bacteria.

ASM Microbe 2019

San Francisco, California, United States

June 20, 2019

Abstract

A predominant limitation in microbiome research is the lack of appropriate and relevant standards to control the technical biases introduced throughout the metagenomics workflow. To address this, ATCC has developed a set of genomic DNA and whole cell mock microbial communities from fully sequenced and characterized ATCC strains that represent species found in the oral, skin, gut, or vaginal microbiome. Here, we demonstrate the utility of these standards as reference materials for 16S and shotgun analyses performed on long-read sequencing platforms. This proof-of-concept analysis demonstrate that ATCC Microbiome Standards are platform agnostic and can be used for the development and optimization of assays performed on both short-read and long-read sequencing platforms.

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Orange-yellow, textured spheres of Clostridium difficile bacteria.

Site-specific microbiome standards

Although there is already a wealth of knowledge on the human microbiome, much of it still remains an enigma. ATCC is committed to supporting this incredible field of research by creating the cutting-edge site-specific standards needed for studies on the oral, skin, gut, and vaginal microbiomes. With these standards, you can expect mock microbial communities comprising fully authenticated and characterized strains representing normal and atypical flora. These standard are ideal for use as controls in microbial profiling of mixed populations or for research on the effects of dysbiosis on human health.

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