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Over the past few decades, much has been written about the misidentification of cell lines, primarily human cell lines. It is not unusual that studies of the ‘same’ cell lines performed by different laboratories often show different results using the same methodology. Thus far, numerous cell lines are known to be misidentified due, in part, to a lack of adequate testing. The financial loss incurred by using misidentified cell lines is estimated in the millions of dollars. Cell line authentication is crucial; however, the scientific community seems indifferent about the consequences or wanting to take steps in preventing it. The validity of scientific data demands that consistent and unequivocal verification of cell line identity is precise. To alleviate these concerns, the implementation of best tissue culture practices to include routine authentication of human cell lines by STR profiling is highly recommended by funding agencies, journal editors, and scientific societies.

Seeing is Believing

Reporter-Labeled Microbial Control Strains

5/21/2015

Experimental controls are essential for establishing that protocols, methods, and instruments are functioning correctly and efficiently. ATCC, a world leader in high-quality biological controls, now offers reporter-labeled microbial control strains, including Escherichia coli, Shiga toxin-producing E. coli (STEC), Pseudomonas aeruginosa, Salmonella enterica, and Shigella flexneri. These labels allow for easy, visual discrimination between control strains and sample strains to minimize errors caused by cross-contamination in the laboratory. In this webinar, we will discuss the development and evaluation of reporter-labeled controls, and will examine their applications in microbial detection and quantification, microscopy, host-pathogen interaction studies, and food testing.

ATCC Transfection Reagents

Powerful Tools to Enable Genetic Manipulation

4/2/2015

Transfection technology allows for transient genetic manipulation in cell cultures that is quick, reliable, and minimally toxic. Lipid-based transfection reagents are powerful tools to investigate the role of various genes in cellular physiology. Gene expression may be enhanced by delivering DNA or mRNA for specific genes or gene products into cells. In addition, gene knockdown may be achieved through transfection of small RNA constructs, which utilize the RNAi pathway to inhibit gene expression. This webinar will provide an introduction to transfection, an overview of current transfection approaches, and a brief guide to best practices in the design and optimization of transfection experiments. Critical aspects include the cell type, culture conditions, and design of nucleic constructs. We will then focus on ATCC’s highly efficient transfection reagents, which have been optimized in a broad spectrum of cell types for a range of applications, including gene expression and siRNA-mediated knockdown.

The quality control microbiology lab serves a valuable function in the pharmaceutical manufacturing environment in terms of providing information on the biological quality of the products being produced. The major tests are familiar to us all – Microbial Limits, Sterility Tests, and Antimicrobial Effectiveness Tests. All of these tests require strict attention to detail for reproducible results, and the compendium also provides guidance on the quality control aspects of laboratory operations. In the first half of this presentation, Dr. Sutton will provide a brief overview of both of these aspects of microbiological quality control with guidance for the listener on how to further research best practice as described in the compendium. In the second half, Mrs. Kerrigan will describe how ATCC is striving to meet the current needs of the quality control microbiology lab.

Vector-borne diseases are a major public health concern, affecting billions of people worldwide. Due to the complexity of vector-borne pathogen transmission, these illnesses are among the most difficult infectious diseases to predict, prevent, and control. Moreover, many vector-borne pathogens can be challenging to culture, require high-containment facilities, or are on the commerce control list, making them difficult to study. To support the development of rapid diagnostic tools and innovative therapeutics, ATCC has synthetically derived nucleic acids that represent key target regions from a number of infectious microorganisms, including dengue virus, West Nile virus, and Eastern equine encephalitis virus, among others. These standards are quantitated, stable, can be handled in BSL-1 conditions, and don’t require permits for international shipping. In this webinar, we will discuss emerging vector-borne microbial pathogens, molecular and biological products from ATCC that support vector-borne research, and will demonstrate the use of ATCC synthetic molecular standards microbial detection and quantification.

Discovering ATCC Hematopoietic Progenitor Cells

Model Systems to Study the Immune and Cardiovascular Systems

2/19/2015

The cellular components of blood originate in bone medullary cavities. In the process of becoming fully functional, hematopoietic cells undergo a program of differentiation which begins in the marrow and may be completed in the peripheral tissues and organs such as blood, lymph, thymus, and spleen. The result is a diversity of cell types, each of which displays specific transport, hemostatic, and immune functions. Hematopoietic research tools have high value for investigating the pathogenesis of anemia and autoimmune diseases, and are useful controls in liquid tumor studies. In this webinar, ATCC scientists will discuss recent developments in developing models of hematopoiesis using immunological cells such as CD34+ bone marrow cells, CD14+ peripheral blood monocytes, and primary peripheral blood mononuclear cells.

Accuracy Matters

Proficiency Testing Programs Offer Dependable Materials for Assessments with Trackable Online Results

2/5/2015

LGC is a leading life sciences measurement and testing business that offers a comprehensive range of products and services, including proficiency testing programs from LGC’s custom-built facility in the north of England. This presentation will discuss the general requirements and purpose of proficiency testing with reference to ISO/IEC 17043:2010. There will also be a brief overview of the different programs and samples offered by LGC, and available from ATCC in the U.S., including program logistics, sample preparation and quality control, and the statistical assessment and reporting of results.

11/13/2014

The emergence of 3D tissue modeling raises new possibilities for the study of complex physiological processes in vitro. Advances in cell isolation, media development, substrates, and growth surfaces are leading to protocols that provide more functionality than traditional 2D cell culture. These models may provide a more predictive analysis and result in a more streamlined process of drug discovery and development. In this webinar, we will discuss recent developments in 3D modeling using ATCC primary and hTERT immortalized cells in areas such as angiogenesis, wound healing, and respiratory studies.

Droplet Digital PCR (ddPCR) is an elegant technology that permits accurate and absolute quantification of target nucleic acid molecules. By subpartitioning a PCR reaction into thousands of nanoliter sized droplets, amplifying, and subsequently individually interrogating them, ddPCR allows for increased quantitative resolution, enhanced detection of difficult to amplify targets, better reproducibility, and simplified data analysis. This webinar will provide a technical overview of ddPCR from technical and workflow related perspectives as well as review key applications such as copy number variation (CNV) analysis, rare mutation detection (RMD) analysis, and standard free (stand-alone) absolute quantification of nucleic acids.

Dopaminergic neurons play significant roles in motor, reward, and motivational behavior related circuits throughout the brain. To date, there are few continuous in vitro models available to laboratories in research, industry, and academia for studies related to basic dopaminergic cell biology or high throughput screening. Here, we propose the use of a human model system, LUHMES cells, to study dopaminergic neuron cell biology. During this webinar, we will highlight some of the advantages of using LUHMES cells, as well as examples of how they have been used in drug screening and to study the molecular mechanisms related to Parkinson’s Disease.