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Cell Culture 101

Tips for Successful Cell Culture

2/9/2017

Time: 12:00 PM Eastern Standard Time

ATCC is widely recognized as the expert in cell culture and production, having the largest supply of cryopreserved cells in the world. In this webinar, we will provide best practices for culturing of cells, from continuous cell lines to primary cells. The information delivered will cover all aspects of successful culture initiation, expansion, authentication, and cryopreservation.

Key Points:

  • ATCC is world renowned as the definitive expert on all aspects of cell culture and production
  • Using misidentified or cross-contaminated cell lines in experiments can invalidate research efforts, therefore authenticating cell lines should be part of your cell culture work flow
  • When culturing specialty cells, such as stem cells or primary cells, certain considerations regarding the choice of media and reagents must be taken

Transforming Molecular Diagnostics

With the GenArraytion MultiFLEX™ Bioassay for Zika, Chikungunya, Dengue, and Yellow Fever Viruses

1/26/2017

Abstract:

Arthropod vectors are responsible for the transmission of some of the most devastating diseases throughout the world. The accurate diagnosis of these diseases can be complicated due to similar clinical presentations, cross-reactivity, or the possibility of co-infection. Thus, there is a need for rapid and accurate methods of detection that can reliably distinguish between causative agents. With the recent outbreak of Zika virus throughout the Americas, it has become imperative to accurately discriminate between this virus and other similar mosquito-borne viruses. In early 2016, GenArraytion developed a MultiFLEX™ Bioassay that identifies six regions of the Zika genome and distinguishes between the Dengue serotypes, Chikungunya, and Yellow Fever viruses as well as Plasmodium falciparum in a single test. In this presentation, we will discuss the caveats of current detection methods, the development of the GenArraytion technology and their speed to market, and how ATCC strains and nucleic acids were used in evaluating the sensitivity and specificity of this assay.

Key Points:

  • There is a need for rapid and accurate methods for detecting and distinguishing between similar mosquito-borne viruses
  • The GenArraytion Mosquito-borne MultiFLEX™ Bioassay is a highly specific and sensitive multiplex PCR assay that can accurately distinguish between Zika, Chikungunya, Dengue, and Yellow Fever viruses within a single sample
  • High-quality, authenticated strains and nucleic acids from ATCC were used to evaluate the sensitivity and specificity of the GenArraytion Mosquito-borne MultiFLEX™ Bioassay

The National Institute of Standards and Technology (NIST) and ATCC® have partnered in an effort to standardize mouse cell line authentication. This webinar will present an overview of the technology requirements, methodology, and reporting the results of the consortium efforts. In addition, an overview of the NIST CRADA was presented.

Mouse Cell Line Authentication Consortium

Abstract: Culturing anaerobes in the laboratory can be challenging. Due to the copious growth requirements and the plethora of anaerobic organisms, knowing the optimal conditions for your anaerobic organism is essential. In this webinar, we will present the various methods to achieve successful growth conditions for a wide variety of anaerobes. We will consider topics such as common anaerobic gas mixtures, media selection, and obtaining anaerobic conditions in the lab. Taking into account individual laboratory constraints, we will also discuss several propagation methods available today. Using specific examples from the ATCC collection, we will demonstrate these techniques.

Key Points:

  • There are numerous types of anaerobic bacteria with various atmospheric and nutritional requirements from clinical gut and wound isolates to environmental extremophiles.
  • Creating and maintaining anaerobic atmospheric and nutritional conditions are critical for the successful growth of anaerobic bacteria.
  • The propagation of anaerobic bacteria has evolved throughout the years. The discovery of new isolates has brought about the need for more robust culturing technology.

Keeping Cells Happy

Topics in Cell Health Maintenance and Viability

9/29/2016

Abstract: Good practices in cell heath are vital to safeguarding the success of long-term cell cultures and obtaining reproducible experimental results. Cell health covers a wide range of topics, from ensuring that your cells are thriving in culture to having confidence that they are characterized correctly. This webinar will explore topics such as cell viability assays that measure growth, authentication of cell lines via short tandem repeat (STR) profiling and mycoplasma detection, as well as aseptic technique and cryopreservation. From services to techniques, we will discuss ATCC’s role in ensuring the health and maintenance of cells in culture.

Key Points:

  • Mycoplasma infection can chronically affect the well-being of cells in culture without being easily detected by visual observation. Specialty kits or routine screening are required to determine if cell cultures are infected.
  • STR profiling detects misidentification or cross-contamination of cell lines, which can invalidate research efforts.
  • When culturing specialty cells, such as stem cells or primary cells, certain considerations regarding the choice of media and reagents must be taken.

ATCC Quantitated Nucleic Acids

Empowering Molecular-based Assay Development

9/22/2016

Abstract: Molecular diagnostics are increasingly relied upon to direct appropriate therapies for the personalized treatment of cancer patients as well as to identify, track, and quantify pathogenic microorganisms. To ensure the reliability and reproducibility of these assays, authenticated controls with confirmed identity, stability, and functionality are required. In this presentation, we will discuss our growing portfolio of quantitative nucleic acids that have been purified or synthetically derived from characterized cell lines and microorganisms. With next-generation sequencing verified gene mutation allelic frequencies and virulence genes, as well as Droplet Digital™ PCR quantified absolute copy numbers, ATCC quantitative nucleic acids provide reliable and sustainable controls for oncology molecular diagnostic assays, infectious disease research, and quality control testing.

Key Points:

  • Human genomic DNAs with known mutational allelic frequency and copy number variation are essential control materials for developing molecular diagnostic tests
  • Quantitative microbial nucleic acids are critical for the development of assays designed for the detection and quantification of pathogenic strains
  • ATCC quantitative nucleic acids are ideal for use in inclusivity/exclusivity testing, establishing limits of detection, and validating or comparing test methods

Abstract: In the food industry, providing safe products and protecting the company brand is of the utmost importance. To help food manufacturers, processors, and contract testing laboratories ensure the safety of consumable goods, ATCC offers an expanding portfolio of quality control strains to help support the routine verification of raw beef manufacturing trimmings for Shiga toxin-producing Escherichia coli (STEC). In this presentation, we will discuss the growing concern on food-borne illnesses, the importance of quality control strains in food safety, and ATCC STEC reference materials that support this need.

Key Points:

  • Food-borne illnesses are a widespread problem that affect tens of millions of people throughout the United States every year
  • ATCC acquires, authenticates, and distributes clinically relevant food-borne pathogens that are essential for use as quality controls in food safety programs
  • Toxigenic, non-toxigenic, and reporter-labeled STEC strains are available from ATCC

Abstract: Angiogenesis is a multi-step physiological process that is involved in a large number of normal and disease state processes. In this webinar, we will introduce the Angio-Ready™ Angiogenesis Assay System, an in vitro co-culture system for measuring angiogenesis, which consists of an assay-ready mixture of an hTERT-immortalized human aortic endothelial cell line (TeloHAEC-GFP) and an hTERT-immortalized adipose-derived mesenchymal stem cell line (hTERT-MSC) in a specially formulated medium. Both cell lines have been highly characterized to verify that they retain the most important characteristics of their parental counterparts. The new co-culture system forms functional tubular structures in less than 7 days and responds appropriately in a dose-dependent manner to known agonists and inhibitors of angiogenesis. Thus, Angio-Ready™ is a ready-to-use, time-saving, high-throughput model for screening drugs or biomolecules for their effect on angiogenesis in cancer, toxicology, diabetes, cardiovascular disease, wound healing, and other pathologies.

Key Points:

  • Angio-Ready™ is a ready-to-use, scalable assay system for angiogenesisis
  • Live imaging is possible with this in vivo-like model consisting of fine tubular structures formed in less than 7 days
  • The formation of vascular tubules responds appropriately to agonists and inhibitors of angiogenesis

    Neural Progenitor Cells

    Models of Toxicology for the 21st Century

    7/28/2016

    Abstract: Human induced pluripotent stem cell-derived neural progenitor cells (NPCs) are an attractive in vitro model to study neurological development, neurotoxicity, and diseases of the nervous system. However, there is a lack of validated NPC lines and media that support differentiation into multiple types of neurons for disease modeling as well as drug and toxicity screening. This webinar will explore the expression of genes associated with the differentiation of NPCs during three weeks in dopaminergic differentiation media. We will then validate that ATCC NPCs and dopaminergic differentiation media are suitable for drug screening in neurotoxicity screenings in NPC-derived neurons by using a viability assay and high-content imaging analysis.

    Key Points:

    • ATCC NPCs cultured in ATCC Dopaminergic Neuron Differentiation Media express a range of specialized neural markers
    • NPCs are sensitive to several compounds known for their cytotoxic effects
    • The complete NPC culture system provides a validated model to investigate the neurotoxicity of a variety of chemotherapeutics

    Discovering ATCC Primary Immune Cells

    Model Systems to Study the Immune and Cardiovascular Systems

    7/14/2016

    Abstract: Hematopoietic stem cells (HSCs) are capable of differentiating into the formed elements of blood: leukocytes, erythrocytes, and thrombocytes. While these distinct cell types display specific immune, transport, and hemostatic phenotypic functions, they are all derived from HSCs. In this webinar we will present data showing that ATCC marrow- and blood-derived primary immune cells, such as bone marrow and cord blood CD34+ cells as well as peripheral blood CD14+ monocytes can be induced to differentiate into various hematopoietic lineages. This webinar will also highlight the use of peripheral blood mononuclear cells to investigate the immunosuppressive capabilities of primary and hTERT-immortalized mesenchymal stem cells in a T-cell proliferation assay.

    Key Points:

    • HSCs are a rapidly advancing area of research producing insights into cellular plasticity, immune function, and disease.
    • Cryopreserved, purified blood cells can be cultured in vitro and induced to differentiate into downstream lineages or used for functional assays.
    • ATCC offers a variety of blood cell type from multiple tissues that are useful in many applications and research areas.