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Time: 12:00 PM Eastern Standard Time

Abstract:

The recent development of the CRISPR/Cas9 system provides a revolutionary gene-editing technology for basic research in biology and for development of targeted cancer therapies. In addition to enabling the identification of novel drug targets through functional screening, CRISPR/Cas9 facilitates the creation of disease models for drug discovery and development.

In this webinar, ATCC experts will address how ATCC utilized this advanced technology to create novel human cell models that contain disease-relevant point mutations and gene rearrangements. In addition, we will introduce a new type of BRAF inhibitor-resistant cell line that was created by using CRISPR/Cas9 to insert the NRAS Q61K mutation. These human isogenic lines provide useful disease models for the identification and validation of new therapeutics.

Key Points:

  • CRISPR/Cas9 gene editing technology is a powerful tool for drug discovery
  • Gene editing technology can be used to create disease-relevant cell models for screening new anti-cancer drug targets
  • CRISPR/Cas9 is a useful tool for creating new types of drug-resistant cell models

Mycoplasma Detection

Protect Your Continuous Cell Cultures

4/20/2017

Time: 12:00 PM Eastern Standard Time

Abstract:

Mycoplasma contamination constitutes a serious concern for cell culturists as it can result in a number of deleterious effects, including the induction of chromosomal abnormalities, the disruption of DNA and RNA synthesis, and the inhibition of both cell metabolism and growth rate. In turn, this can affect assay reproducibility, compromise data validity, and lead to the misinterpretation of results. To minimize these risks, routine testing of cell cultures and reagents is recommended. In this presentation, we will discuss the most frequently used mycoplasma testing methods, and will expand on the products and services offered by ATCC that support the early detection of mycoplasma as well as the development and validation of novel test methods.

Key Points:

  • Mycoplasma contamination can affect the phenotypic and functional characteristics of cells in vitro, compromising the validity of generated data
  • The best protection against mycoplasma is to use proper aseptic technique and to quickly identify contaminated cultures and reagents through routine testing
  • Common methods of mycoplasma detection include direct culture, Hoechst DNA staining, and PCR-based testing

ATCC Microbiology

Best Practices for Stock Maintenance

5/18/2017

Time: 12:00 PM Eastern Standard Time

Abstract:

Authenticated reference materials are essential for assay reproducibility and data integrity. To ensure the quality of these cultures, it is imperative that they are well-characterized and carefully managed through preservation and storage protocols that maintain the genotype and phenotype. In this webinar, we will discuss the best practices for stock maintenance with regard to passage, storage, recovery, and microbial authentication, and how ATCC manages these through the seed stock concept, adhering to the specific needs of each culture, and polyphasic strain authentication.

Key Points:

  • Minimizing passage number reduces the likelihood of contamination, genetic drift, mutation, and phenotypic variation
  • Proper storage and recovery of frozen and freeze-dried cultures protects post-preservation viability
  • Combining phenotypic, genotypic, proteotypic, and functional analyses provides the best possible strain authentication and characterization

ATCC Biorepository Services℠

Processing of Biospecimens in a Controlled Environment

5/25/2017

Time: 12:00 PM Eastern Standard Time

Abstract:

Biological materials produced through scientific inquiry are often valuable assets that support important scientific breakthroughs. To ensure the safety and future availability of these resources, they should be stored within a biorepository to protect against loss, contamination, or genetic drift. In this webinar, we will discuss the importance of biological materials management and will expand on the services offered at ATCC that support the handling and storage of biological specimens

Key Points:

  • Biobanking of cell lines, microbial strains, and other biological reagents generated through scientific investigation with a biorepository ensures they will be accessible to support future scientific endeavors
  • ATCC Biorepository Services℠ delivers secure and reliable biological material management with temperature-controlled supply chain, 24/7 equipment monitoring, and on-call after-hours personnel
  • ATCC provides extensive support to customers needing cGMP compliant and non-cGMP master cell banks and working cell banks, including short- and long-term storage of small- or large-scale specimens