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Abstract: Culturing anaerobes in the laboratory can be challenging. Due to the copious growth requirements and the plethora of anaerobic organisms, knowing the optimal conditions for your anaerobic organism is essential. In this webinar, we will present the various methods to achieve successful growth conditions for a wide variety of anaerobes. We will consider topics such as common anaerobic gas mixtures, media selection, and obtaining anaerobic conditions in the lab. Taking into account individual laboratory constraints, we will also discuss several propagation methods available today. Using specific examples from the ATCC collection, we will demonstrate these techniques.

Key Points:

  • There are numerous types of anaerobic bacteria with various atmospheric and nutritional requirements from clinical gut and wound isolates to environmental extremophiles.
  • Creating and maintaining anaerobic atmospheric and nutritional conditions are critical for the successful growth of anaerobic bacteria.
  • The propagation of anaerobic bacteria has evolved throughout the years. The discovery of new isolates has brought about the need for more robust culturing technology.

Angio-Ready

An improved in vitro model of angiogenesis

6/8/2017

Time: 12:00 PM Eastern Standard Time

Abstract:

Angiogenesis is a multi-step physiological process that is involved in a large number of normal and disease state processes. In this webinar, we will introduce the Angio-Ready™ Angiogenesis Assay System, an in vitro co-culture system for measuring angiogenesis. Angio-Ready™ consists of an assay-ready mixture of an hTERT-immortalized human aortic endothelial cell line (TeloHAEC-GFP) and an hTERT-immortalized adipose-derived mesenchymal stem cell line (hTERT-MSC) in a specially formulated medium. Both cell lines have been well-characterized to verify that they retain the most important characteristic of their parental counterparts. The new co-culture system forms functional tubular structures in less than 7 days and responds appropriately in a dose dependent manner to known agonists and inhibitors of angiogenesis. Thus, Angio-Ready™ is a ready-to-use, time-saving, high-throughput model for screening drugs or biomolecules for their effect on angiogenesis in cancer, toxicology, diabetes, cardiovascular disease, wound healing, and other pathologies.

Key Points:

  • Angio-Ready™ is a ready-to-use, scalable assay system for angiogenesis
  • Live imaging is possible with this in vivo-like model consisting of fine tubular structures formed in less than 7 days
  • The formation of vascular tubules responds appropriately to agonists and inhibitors of angiogenesis

ATCC Biorepository Services℠

Processing of Biospecimens in a Controlled Environment

5/25/2017

Time: 12:00 PM Eastern Standard Time

Abstract:

Biological materials produced through scientific inquiry are often valuable assets that support important scientific breakthroughs. To ensure the safety and future availability of these resources, they should be stored within a biorepository to protect against loss, contamination, or genetic drift. In this webinar, we will discuss the importance of biological materials management and will expand on the services offered at ATCC that support the handling and storage of biological specimens

Key Points:

  • Biobanking of cell lines, microbial strains, and other biological reagents generated through scientific investigation with a biorepository ensures they will be accessible to support future scientific endeavors
  • ATCC Biorepository Services℠ delivers secure and reliable biological material management with temperature-controlled supply chain, 24/7 equipment monitoring, and on-call after-hours personnel
  • ATCC provides extensive support to customers needing cGMP compliant and non-cGMP master cell banks and working cell banks, including short- and long-term storage of small- or large-scale specimens

ATCC Microbiology

Best Practices for Stock Maintenance

5/18/2017

Time: 12:00 PM Eastern Standard Time

Abstract:

Authenticated reference materials are essential for assay reproducibility and data integrity. To ensure the quality of these cultures, it is imperative that they are well-characterized and carefully managed through preservation and storage protocols that maintain the genotype and phenotype. In this webinar, we will discuss the best practices for stock maintenance with regard to passage, storage, recovery, and microbial authentication, and how ATCC manages these through the seed stock concept, adhering to the specific needs of each culture, and polyphasic strain authentication.

Key Points:

  • Minimizing passage number reduces the likelihood of contamination, genetic drift, mutation, and phenotypic variation
  • Proper storage and recovery of frozen and freeze-dried cultures protects post-preservation viability
  • Combining phenotypic, genotypic, proteotypic, and functional analyses provides the best possible strain authentication and characterization

Mycoplasma Detection

Protect Your Continuous Cell Cultures

4/20/2017

Time: 12:00 PM Eastern Standard Time

Abstract:

Mycoplasma contamination constitutes a serious concern for cell culturists as it can result in a number of deleterious effects, including the induction of chromosomal abnormalities, the disruption of DNA and RNA synthesis, and the inhibition of both cell metabolism and growth rate. In turn, this can affect assay reproducibility, compromise data validity, and lead to the misinterpretation of results. To minimize these risks, routine testing of cell cultures and reagents is recommended. In this presentation, we will discuss the most frequently used mycoplasma testing methods, and will expand on the products and services offered by ATCC that support the early detection of mycoplasma as well as the development and validation of novel test methods.

Key Points:

  • Mycoplasma contamination can affect the phenotypic and functional characteristics of cells in vitro, compromising the validity of generated data
  • The best protection against mycoplasma is to use proper aseptic technique and to quickly identify contaminated cultures and reagents through routine testing
  • Common methods of mycoplasma detection include direct culture, Hoechst DNA staining, and PCR-based testing

Time: 12:00 PM Eastern Standard Time

Abstract:

The recent development of the CRISPR/Cas9 system provides a revolutionary gene-editing technology for basic research in biology and for development of targeted cancer therapies. In addition to enabling the identification of novel drug targets through functional screening, CRISPR/Cas9 facilitates the creation of disease models for drug discovery and development.

In this webinar, ATCC experts will address how ATCC utilized this advanced technology to create novel human cell models that contain disease-relevant point mutations and gene rearrangements. In addition, we will introduce a new type of BRAF inhibitor-resistant cell line that was created by using CRISPR/Cas9 to insert the NRAS Q61K mutation. These human isogenic lines provide useful disease models for the identification and validation of new therapeutics.

Key Points:

  • CRISPR/Cas9 gene editing technology is a powerful tool for drug discovery
  • Gene editing technology can be used to create disease-relevant cell models for screening new anti-cancer drug targets
  • CRISPR/Cas9 is a useful tool for creating new types of drug-resistant cell models

Time: 12:00 PM Eastern Standard Time

Abstract:

Assay consistency and standardization are essential for providing the best patient care. To help promote the generation of reliable and reproducible data, the use of authenticated, well-characterized standards are needed throughout assay development, validation, and implementation. In this webinar, we will discuss the need for standards in clinical laboratory testing, and we will expand on how ATCC supports this with authenticated biological and molecular reference materials.

Key Points:

  • There is a recognized need for established, fully characterized, globally accepted reference materials
  • Standards can be used to benchmark critical assay performance during development, validation, and implementation
  • ATCC supports scientific research and breakthroughs through the continual development of authenticated standards and reference materials

Finding Your Perfect Match

Evolving Technologies for Bacterial Strain Typing

3/9/2017

Time: 12:00 PM Eastern Standard Time

Abstract:

Bacterial strains within the same species can show a wide range of genetic differences, from only a few nucleotides to large chromosomal variations. Identifying specific strains can provide important clues to antimicrobial resistance or virulence factors, and is central to epidemiological studies of disease outbreaks. In this webinar, we will look at the variety of typing information used to identify strains in the ATCC catalog. From traditional serological methods to various electrophoresis-based molecular methods and the latest applications of whole genome sequencing, we will discuss these technologies, their applications in current literature, and how ATCC is working to improve strain typing by providing quality control and reference materials.

Key Points

  • The amount and type of strain difference within a given species of bacteria can differ widely and has given rise to a variety of strain typing technologies
  • Molecular methods have become predominant and have evolved from primarily electrophoresis-based methods, such as pulsed-field gel electrophoresis, to sequence-based methods, such as those based on one or more loci
  • Whole genome sequencing offers great promise for future typing as it offers both high resolution and the power of examining many loci, but comes with challenges in terms of quality control and data management

2/23/2017

Time: 12:00 PM Eastern Standard Time

Abstract:

In vivo studies have shown that kidney membrane transporters play a key part in drug disposition and renal clearance. Primary renal proximal tubule epithelial cells (RPTEC) are the most physiologically relevant cell models, but lose OAT1, OCT2, and OAT3 transporter expression in culture. In addition, primary RPTEC transiently expressing these transporters show large variations between production lots, making the data hard to interpret. Furthermore, cell line-based models either do not have the kidney tissue origination or the cell line itself is a cancer line.

This presentation will introduce transporter cell models using a well characterized hTERT-immortalized RPTEC that stably overexpress either the OAT1, OCT2, and OAT3 gene. Our data show that these modified cell lines are very useful tools that provide kidney tissue-relevant results, improved consistency over time, and more predictability for clinical trials versus current models.

Key Points:

  • Kidney membrane transporters play a key part in drug disposition and renal clearance, however there is a lack of in vitro models that durably and correctly recapitulate kidney physiology
  • ATCC has created kidney cell models using a well characterized hTERT-immortalized RPTEC that stably overexpress the OAT1, OCT2, or OAT3 gene
  • Our data shows that these modified cell lines are very useful tools that provide kidney tissue-relevant results, improved consistency over time, and predictability for clinical trials

Cell Culture 101

Tips for Successful Cell Culture

2/9/2017

Time: 12:00 PM Eastern Standard Time

ATCC is widely recognized as the expert in cell culture and production, having the largest supply of cryopreserved cells in the world. In this webinar, we will provide best practices for culturing of cells, from continuous cell lines to primary cells. The information delivered will cover all aspects of successful culture initiation, expansion, authentication, and cryopreservation.

Key Points:

  • ATCC is world renowned as the definitive expert on all aspects of cell culture and production
  • Using misidentified or cross-contaminated cell lines in experiments can invalidate research efforts, therefore authenticating cell lines should be part of your cell culture work flow
  • When culturing specialty cells, such as stem cells or primary cells, certain considerations regarding the choice of media and reagents must be taken