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Transforming Molecular Diagnostics

With the GenArraytion MultiFLEX™ Bioassay for Zika, Chikungunya, Dengue, and Yellow Fever Viruses

1/26/2017

Abstract:

Arthropod vectors are responsible for the transmission of some of the most devastating diseases throughout the world. The accurate diagnosis of these diseases can be complicated due to similar clinical presentations, cross-reactivity, or the possibility of co-infection. Thus, there is a need for rapid and accurate methods of detection that can reliably distinguish between causative agents. With the recent outbreak of Zika virus throughout the Americas, it has become imperative to accurately discriminate between this virus and other similar mosquito-borne viruses. In early 2016, GenArraytion developed a MultiFLEX™ Bioassay that identifies six regions of the Zika genome and distinguishes between the Dengue serotypes, Chikungunya, and Yellow Fever viruses as well as Plasmodium falciparum in a single test. In this presentation, we will discuss the caveats of current detection methods, the development of the GenArraytion technology and their speed to market, and how ATCC strains and nucleic acids were used in evaluating the sensitivity and specificity of this assay.

Key Points:

  • There is a need for rapid and accurate methods for detecting and distinguishing between similar mosquito-borne viruses
  • The GenArraytion Mosquito-borne MultiFLEX™ Bioassay is a highly specific and sensitive multiplex PCR assay that can accurately distinguish between Zika, Chikungunya, Dengue, and Yellow Fever viruses within a single sample
  • High-quality, authenticated strains and nucleic acids from ATCC were used to evaluate the sensitivity and specificity of the GenArraytion Mosquito-borne MultiFLEX™ Bioassay

Cell Culture 101

Best Practices for Stock Maintenance

2/9/2017

ATCC is widely recognized as the expert in cell culture and production, having the largest supply of cryopreserved cells in the world. In this webinar, we will provide best practices for culturing of cells, from continuous cell lines to primary cells. The information delivered will cover all aspects of successful culture initiation, expansion, authentication, and cryopreservation.

Key Points:

  • ATCC is world renowned as the definitive expert on all aspects of cell culture and production
  • Using misidentified or cross-contaminated cell lines in experiments can invalidate research efforts, therefore authenticating cell lines should be part of your cell culture work flow
  • When culturing specialty cells, such as stem cells or primary cells, certain considerations regarding the choice of media and reagents must be taken

Registration for this session is coming soon.

Development of Solute Carrier Transporters Kidney Cell Models

Using hTERT-immortalized Renal Proximal Tubule Epithelial Cells

2/23/2017

Abstract:

In vivo studies have shown that kidney membrane transporters play a key part in drug disposition and renal clearance. Primary renal proximal tubule epithelial cells (RPTEC) are the most physiologically relevant cell models, but lose OAT1, OCT2, and OAT3 transporter expression in culture. In addition, primary RPTEC transiently expressing these transporters show large variations between production lots, making the data hard to interpret. Furthermore, cell line-based models either do not have the kidney tissue origination or the cell line itself is a cancer line.

This presentation will introduce transporter cell models using a well characterized hTERT-immortalized RPTEC that stably overexpress either the OAT1, OCT2, and OAT3 gene. Our data show that these modified cell lines are very useful tools that provide kidney tissue-relevant results, improved consistency over time, and more predictability for clinical trials versus current models.

Key Points:

  • Kidney membrane transporters play a key part in drug disposition and renal clearance, however there is a lack of in vitro models that durably and correctly recapitulate kidney physiology
  • ATCC has created kidney cell models using a well characterized hTERT-immortalized RPTEC that stably overexpress the OAT1, OCT2, or OAT3 gene
  • Our data shows that these modified cell lines are very useful tools that provide kidney tissue-relevant results, improved consistency over time, and predictability for clinical trials

Registration for this session is coming soon.

Finding Your Perfect Match

Evolving Technologies for Bacterial Strain Typing

3/9/2017

Abstract:

Bacterial strains within the same species can show a wide range of genetic differences, from only a few nucleotides to large chromosomal variations. Identifying specific strains can provide important clues to antimicrobial resistance or virulence factors, and is central to epidemiological studies of disease outbreaks. In this webinar, we will look at the variety of typing information used to identify strains in the ATCC catalog. From traditional serological methods to various electrophoresis-based molecular methods and the latest applications of whole genome sequencing, we will discuss these technologies, their applications in current literature, and how ATCC is working to improve strain typing by providing quality control and reference materials.

Key Points

  • The amount and type of strain difference within a given species of bacteria can differ widely and has given rise to a variety of strain typing technologies
  • Molecular methods have become predominant and have evolved from primarily electrophoresis-based methods, such as pulsed-field gel electrophoresis, to sequence-based methods, such as those based on one or more loci
  • Whole genome sequencing offers great promise for future typing as it offers both high resolution and the power of examining many loci, but comes with challenges in terms of quality control and data management

Registration for this session is coming soon.

Mycoplasma Detection

Protect Your Continuous Cell Cultures

4/20/2017

Abstract:

Mycoplasma contamination constitutes a serious concern for cell culturists as it can result in a number of deleterious effects, including the induction of chromosomal abnormalities, the disruption of DNA and RNA synthesis, and the inhibition of both cell metabolism and growth rate. In turn, this can affect assay reproducibility, compromise data validity, and lead to the misinterpretation of results. To minimize these risks, routine testing of cell cultures and reagents is recommended. In this presentation, we will discuss the most frequently used mycoplasma testing methods, and will expand on the products and services offered by ATCC that support the early detection of mycoplasma as well as the development and validation of novel test methods.

Key Points:

  • Mycoplasma contamination can affect the phenotypic and functional characteristics of cells in vitro, compromising the validity of generated data
  • The best protection against mycoplasma is to use proper aseptic technique and to quickly identify contaminated cultures and reagents through routine testing
  • Common methods of mycoplasma detection include direct culture, Hoechst DNA staining, and PCR-based testing

Registration for this session is coming soon.

ATCC Microbiology

Best Practices for Stock Maintenance

5/18/2017

Abstract:

Authenticated reference materials are essential for assay reproducibility and data integrity. To ensure the quality of these cultures, it is imperative that they are well-characterized and carefully managed through preservation and storage protocols that maintain the genotype and phenotype. In this webinar, we will discuss the best practices for stock maintenance with regard to passage, storage, recovery, and microbial authentication, and how ATCC manages these through the seed stock concept, adhering to the specific needs of each culture, and polyphasic strain authentication.

Key Points:

  • Minimizing passage number reduces the likelihood of contamination, genetic drift, mutation, and phenotypic variation
  • Proper storage and recovery of frozen and freeze-dried cultures protects post-preservation viability
  • Combining phenotypic, genotypic, proteotypic, and functional analyses provides the best possible strain authentication and characterization

Registration for this session is coming soon.