Vaccinia virus

VR-1354 ^™

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Vaccinia virus strain WR is a tissue culture-adapted strain that was derived from Vaccine virus strain WR (ATCC VR-119) via multiple laboratory passages in cell culture. This preparation has been shown to serve as an effective expression vector for foreign genes and has been widely distributed and used in constructing vectors for gene expression.

This product requires a VS 16-6A permit, which can be downloaded at the USDA website here. Please submit the completed permit form to [email protected] with your ATCC sales order number and/or your purchase number noted in the subject line and body of the email.

Product category: Viruses
Product type: Animal virus
Classification: Poxviridae, Orthopoxvirus
Strain designation: WR (NIH TC-adapted)
Genome sequenced strain: Yes
Isolation source: Multiple laboratory passages in cell culture of WR strain of vaccinia.
Product format: Frozen
Storage conditions: -70°C or colder

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Documentation

Product sheet

BSL 2

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Required Products

These products are vital for the proper use of this item and have been confirmed as effective in supporting functionality. If you use alternative products, the quality and effectiveness of the item may be affected.

Vero

CCL-81

Eagle's Minimum Essential Medium (EMEM)

30-2003

Fetal Bovine Serum (FBS)

30-2020

General

Specific applications: testing disinfectants

Characteristics

Comments

This strain is recommended by ATCC for use in the tests described in Fed. Spec. A-A-1438 where only the taxon is specified.

This tissue culture adapted WR strain of vaccinia is derived from ATCC VR-119 and has been shown to serve as an effective expression vector for foreign genes (Mackett et al., J. Virol., 1984)

Dr. Moss received the WR strain in 1966 from Dr. Salzman who obtained the virus as ATCC VR-119 from the ATCC. The tissue culture adapted strain WR has been widely distributed and used in constructing vectors for gene expression (J. Gen Virol, 1986).

Vaccination recommended for lab staff manipulating vaccinia, monkeypox, or cowpox viruses (CDC/NIH Biosafety in Microbiological and Biomedical Laboratories, 4th ed, 1999 - stock no. 017-040-00547-4).

Cell associated virus from infected HeLa cells

Mycoplasma contamination

Not detected

Handling information

Host: Vero (ATCC CCL-81)
Effects: cell rounding; cell sloughing; CPE
Complete medium: EMEM (ATCC 30-2003) + 2% FBS (ATCC 30-2020)
Temperature: 37°C
Atmosphere: 95% Air, 5% CO₂
Incubation: Incubate infected culture for 2-3 days at 37°C in a humidified 5% CO₂ atmosphere, until CPE are well advance throughout.
Recommendation for infection: Plate cells 24 hours prior to infection and infect when cultures are 70-80% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm²) diluted in Virus growth medium: EMEM to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO₂ atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Handling notes: Vaccination is recommended for lab staff manipulating vaccinia, monkeypox, or cowpox viruses. This TC-adapted WR strain of vaccinia is derived from ATCC VR-119 and has been shown to serve as an effective expression vector for foreign genes.
Key abbreviations: °C, Degrees Celsius

CO₂, Carbon dioxide

CPE, Cytopathic effect

EMEM, Eagle's Minimum Essential Medium

FBS, Fetal bovine serum

MOI, Multiplicity of infection

NCBI, National Center for Biotechnology Information

NIH, National Institutes of Health

Quality control specifications

Verification method: Whole-genome Sequencing

History

Deposited as: Vaccinia virus, Orthopoxvirus
Depositors: NS Cooper, B Moss, NIH
Cross references: GenBank AY243312.1 Vaccinia virus WR, complete genome

GenBank S64006 E3L=double-stranded RNA-binding protein [vaccinia virus, WR, ATCC VR1354, Genomic, 633 nt].

GenBank NC_006998.1 Vaccinia virus, complete genome

Legal disclaimers

Intended use

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

Warranty

The product is provided 'AS IS' and the viability of ATCC^® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

United States Veterinary Permit for Importation and Transportation of Controlled Materials and Organisms and Vectors

For every order of this item, you must provide a valid Permit for Importation and Transportation of Controlled Materials and Organisms and Vectors (VS Form 16-6A) obtained from the United States Department of Agriculture (USDA), Animal and Plant Health Inspection Service. We cannot ship this item until we receive this permit.

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Salzman NP, Sebring ED. Sequential formation of vaccinia virus proteins and viral deoxyribonucleic acid replication. J. Virol. 1: 16-23, 1967. PubMed: 4248263

Mackett M, et al. Vaccinia virus: a selectable eukaryotic cloning and expression vector. Proc. Natl. Acad. Sci. USA 79: 7415-7419, 1982. PubMed: 6296831

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