Human echovirus 1 (ATCC® VR-1808)

Classification: Picornaviridae, Enterovirus, Enterovirus B  /  Product Format: frozen 1.0 mL per vial

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Classification Picornaviridae, Enterovirus, Enterovirus B
Agent Human echovirus 1
Strain Farouk
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 1.0 mL per vial
Storage Conditions -70°C or colder
Images ATCCC VR-1808 Micrograph
Comments This item has been treated with diethyl ether to remove Mycoplasma at the ATCC. The previous item number was VR-31™

Monkeys and rabbits yield satisfactory immune sera.  No apparent infection in monkeys, rabbits or newborn mice.  Cross-reacts antigenically with Echovirus 8.
Effect on Host
CPE, rounding and sloughing
Recommended Host
LLC-MK2 cells (ATCC® CCL-7.1™)
Growth Conditions Temperature: 37°C
Recommendations for Infection: For best results, infection should be performed on an 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2), optimized for propagation (e.g. MOI 0.01-0.1 recommended). Wash monolayer two times with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 2-5 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.
Effect on Host
CPE, rounding and sloughing
Name of Depositor JL Melnick, Yale University School of Medicine
Source Feces from normal infant, Egypt, 1951
Year of Origin 1951

Melnick JL, Agren K. Poliomyelitis and Coxsackie viruses isolated from normal infants in Egypt. Proc. Soc. Exp. Biol. Med. 81: 621-624, 1952. PubMed: 13037743

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These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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