Depositors: R Weiss, Massachusetts Institute of Technology

Permits and Restrictions

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Designations pENTR_L4R1_pCYC1MIN
Depositors R Weiss, Massachusetts Institute of Technology
Applications An entry vector carrying a very weak promoter for gene expression in Saccharomyces cerevisiae
Vector Total size of construct (kb): 2.925
Vector type: Entry vector
Markers: kanR
Insert Insert size (Kb): 0.15
Suggested primers for sequencing:
M13 Forward: 5’- GTTTTCCCAGTCACGAC - 3’
M13 Reverse: 5’- CAGGAAACAGCTATGAC - 3’
Before opening the vial, centrifuge at 6,000 x g for 30 seconds. Add 30 µL of Molecular Grade Water and incubate the vial at 4°C overnight to dissolve the DNA. Each vial contains 2-3 µg plasmid DNA (measured by PicoGreen® dsDNA quantitation assay).
Related Products ATCC SB-1008
ATCC SB-1035
ATCC SB-1036
ATCC SB-1038
ATCC SB-1041
ATCC SB-1044
ATCC SB-1105
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

This construct contains the minimal yeast CYC1 promoter (146 bp) flanked by two att sites (att L4 and att R1).