Sporolactobacillus kofuensis Yanagida et al.

1. Open vial according to enclosed instructions or visit www.atcc.org for instructions.
2. Rehydrate the entire pellet with approximately 0.5 mL of #2847 broth. Aseptically transfer the entire contents to a 5-6 mL tube of #2847 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary tubes.
3. Use several drops of the primary broth tube to inoculate a #2847 plate and/or #2847 agar slant.
4. Incubate at 30°C for 48 to 96 hours.

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