CellMatrix Basement Membrane Gel (ATCC® ACS-3035)

Product Format frozen 5 mL
Storage Conditions -80°C or colder
Applications
  • Three-dimensional culture of primary tissue derived human and mouse organoids
  • Three-dimensional culture of iPSC derived organoids
  • Three-dimensional culture of tumorspheres from various cell lines
  • Sandwich culture of human hepatocytes
  • Feeder-free culture of human embryonic stem cells (hESC) and induced pluripotent stem cells (hiPSC)
  • Culture of iPSCs-derived neural progenitor cells (NPCs) and neurons
  • Angiogenesis modeling via the tube formation assay
Comments
Each lot of CellMatrix Gel is shown to promote the attachment of hESCs/hiPSCs and to maintain hESCs/hiPSCs in a pluripotent state as determined by the expression of Oct-4 and Nanog pluripotent markers.
Store at -80°C in aliquots. Avoid repeated freezing and thawing.
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Quality Control Specifications

Functional testing: Each lot of CellMatrix Gel is shown to promote the attachment of hESCs/hiPSCs and to maintain hESCs/hiPSCs in a pluripotent state as determined by the expression of Oct-4 and Nanog pluripotent markers.

Sterility testing: No bacterial or fungal growth is detected. Negative for the presence of mycoplasma and 31 pathogens and viruses, including LDEV, as demonstrated by PCR. Endotoxin concentrations are less than 8 EU/mL by LAL assay. 

Concentration: 12 to 18 mg/mL. Please refer to the certificate of analysis for the lot-specific concentration. 

Storage Buffer: Product is stored in Dulbecco’s Modified Eagle’s Medium (DMEM) with 10 µg/mL gentamycin, without phenol red. A Certificate of Analysis is available upon request.

Sterility Testing
No bacterial or fungal growth is detected. Negative for the presence of mycoplasma and 31 pathogens and viruses, including LDEV, as demonstrated by PCR. Endotoxin concentrations are less than 8 EU/mL by LAL assay.
Basic Documentation
Other Documentation
References

Bergqvist C, et al. An inner nuclear membrane protein induces rapid differentiation of human induced pluripotent stem cells. Stem Cell Res 23:33-38, 2017. PubMed: 28668644