Rickettsia tamurai (ATCC® VR-1594)

Classification: Rickettsiaceae, Rickettsieae  /  Product Format: frozen

Classification Rickettsiaceae, Rickettsieae
Agent Rickettsia tamurai
Strain AT-1
Positive for Mycoplasma Yes
Biosafety Level 3

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Storage Conditions -80°C or colder  
Comments
The AT-1 strain is recognized as the type strain for a newly identified rickettsial species (Rickettsia tamurae), a gram -negative, obligately intracellular and non-motile bacterium.
The preparation available through ATCC tested positive for mycoplasma (Mycoplasma orale) contamination by PCR.
Requests for this agent require special permits and a signed statement assuming all risks and responsibility for laboratory handling.
Effect on Host
Bacteria visible in cells after staining with Gimenez stain.
Recommended Host
Vero cells (ATCC CRL-1587)
Growth Conditions
Recommendations for Infection: 5 days at 32°C, a 5% CO2 in air atmosphere is recommended in EMEM (ATCC 30-2003); with 2mM L-glutamine supplemented with 2% FBS (ATCC 30-2020), following centrifugation at 700 g for one hour at 22°C
Effect on Host
Bacteria visible in cells after staining with Gimenez stain.
Name of Depositor Dr. P. -E. Fournier
Source
Isolated from Amblyomma testudinarium nymph in Anan, Tokushima prefecture, Japan, in 1993
References

Marrero, M and Raoult D. Centrifugation-shell vial technique for rapid detection of Mediterranean spotted fever rickettsia in blood culture. Am J Trop Med Hyg. 1989 Feb;40(2):197-9. PubMed: 2645804

Fournier, P. E. et. al. Rickettsia tamurae sp. nov., isolated from Amblyomma testudinarium ticks. Int J Syst Evol Microbiol. 2006 Jul;56(Pt 7):1673-75. PubMed: 16825648