Avian reovirus type 3 (ATCC® VR-857)

Classification: Reoviridae, Reovirus  /  Product Format: frozen

Classification Reoviridae, Reovirus
Agent Avian reovirus type 3
Strain 59
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
Intestine from chicken with cloacal pasting
Product Format frozen
Storage Conditions  -70°C or colder
Comments
Deposited as Avian reovirus type 3. Pathogenic for newborn mice.
Effect on Host
CPE, syncytia formation
 CPE, syncytia formation
Recommended Host
Primary chicken embryo fibroblasts

Production Host:  Primary CEF

Growth Conditions
Temperature: 37°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 2 to 7 days in a humidified 5% CO2 atmosphere
Effect on Host
CPE, syncytia formation
 CPE, syncytia formation
Name of Depositor BL Patel, University of Minnesota, College of Veterinary Medicine
Source
Intestine from chicken with cloacal pasting
References

Deshmukh DR, Pomeroy BS. Avian reoviruses. I. Isolation and serological characterization. Avian Dis. 13: 239-243, 1969. PubMed: 4307737