Avian reovirus type 3 (ATCC® VR-857)

Classification: Reoviridae, Reovirus  /  Product Format: frozen

Permits and Restrictions

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Classification Reoviridae, Reovirus
Agent Avian reovirus type 3
Strain 59
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Intestine from chicken with cloacal pasting
Product Format frozen
Storage Conditions  -70°C or colder
Deposited as Avian reovirus type 3. Pathogenic for newborn mice.
Effect on Host
CPE, syncytia formation
 CPE, syncytia formation
Recommended Host
Primary chicken embryo fibroblasts

Production Host:  Primary CEF

Growth Conditions
Temperature: 37°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 2 to 7 days in a humidified 5% CO2 atmosphere
Effect on Host
CPE, syncytia formation
 CPE, syncytia formation
Name of Depositor BL Patel, University of Minnesota, College of Veterinary Medicine
Intestine from chicken with cloacal pasting

Deshmukh DR, Pomeroy BS. Avian reoviruses. I. Isolation and serological characterization. Avian Dis. 13: 239-243, 1969. PubMed: 4307737

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.
  • Customer Acceptance of Responsibility, ATCC Form 62 required for distribution.
  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation