Trypanosoma cruzi Chagas (ATCC® 50798)

Strain Designations: I/INF/BO/80/SC43 CL1  /  Depositor: JA Dvorak  /  Biosafety Level: 2

Strain Designations I/INF/BO/80/SC43 CL1
Vector borne research
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Triatoma infestans, Bolivia, 1980, cloned by M. Tibayrenc, 1980
Product Format frozen
Type Strain no
Brazilian and Bolivian zymodemes
isoenzyme profile
Medium ATCC® Medium 1029: LIT medium
Growth Conditions
Temperature: 25.0°C
Duration: axenic
1.   Harvest cells from several cultures in very late logarithmic to early stationary phase of growth.  Vigorously agitate to suspend the cells.

2.   Aseptically transfer the cell suspension to 15 ml plastic centrifuge tubes.

3.   Centrifuge at ~800 x g for 5 min.

4.   While cells are centrifuging, prepare a 10% solution of DMSO in complete ATCC Medium 1029.  Cool on ice.

5.   Remove the supernatant and pool the cell pellets to the final volume desired with fresh growth medium.

6.   Combine the cell suspension with an equal volume of 10% DMSO cryoprotectant solution (prepared in step 4) to yield a final concentration of 5% DMSO.

7.   Dispense in 0.5 ml aliquots to 1.0-2.0 ml Nunc vials (special plastic vials for cryopreservation).

8.   Place the vials in a controlled rate freezing unit.  From room temperature cool at -1°C/min to -40°C.  At -40°C, plunge ampules into liquid nitrogen.  Alternatively, place the vials in a Nalgene 1°C freezing apparatus.  Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen. (The cooling rate in this apparatus is approximately -1°C/min.).  

9.   Store ampules in a liquid nitrogen refrigerator until needed.

10.          To establish a culture from the frozen state, place a frozen ampule in a 35°C water bath just enough to cover the frozen material.  Allow the ampule to thaw completely (2-3 min).

11.          Immediately after thawing, aseptically remove the contents and transfer to a T-25 tissue culture flask containing 10 ml of fresh complete ATCC medium 1029.

12.Screw the cap on tightly and incubate at 20-25°C.    Observe the culture daily and transfer when numerous trophozoites are observed.         

Name of Depositor JA Dvorak
Special Collection NCRR Contract
Chain of Custody
ATCC <<--JA Dvorak<<--M.A. Miles
Year of Origin 1980

Chapman MD, et al. Trypanosoma cruzi from the Paraguayan Chaco: isoenzyme profiles of strains isolated at Makthlawaiya. J. Protozool. 31: 482-486, 1984. PubMed: 6239030