pK (ATCC® 77380)

Applications: expression vectorvector permitting construction of fusion proteinsvector permitting visual detection of recombinants  /  Depositors: JJ McCormick

Permits and Restrictions

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Designations pK
Depositors JJ McCormick
Biosafety Level 1
Vector Information
Size (kb): 2.7660000324249270
Vector: pK (plasmid)
Promoters: Promoter lac
Construction: pUC19
Marker(s):ampR
Construct size (kb): 2.766000032424927
Features: insert detection: lacZ'
marker(s): ampR
promoter: lac
replicon: pMB1
Applications
expression vector
vector permitting construction of fusion proteins
vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): PvuI--1.8, 1.0; EcoRI--2.8; HindIII--2.8; SspI--2.8; ClaI--2.8.
The plasmid contains the following restriction sites (bp from 0): NotI--0; Nsi--75; PvuI--119, 1907; EcoRI--237; HindIII--288; SapI--524; DrdI--749, 2618; BsaI--1607; SspI--2342.
Cloning vector with a second multiple cloning site inserted at the KasI site of pUC19 not interrupting lacZ'.
The order of the major features in this plasmid is: NotI/MCSII/NsiI - 3' lacZ' - EcoRI/MCSI/HindIII - 5' lacZ' - SapI - pMB1 ori - ampR.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Wang Q, et al. Mammalian expression vectors with modulatable promoters and two multiple cloning sites. Gene 119: 155-161, 1992. PubMed: 1327962

J J McCormick, personal communication

Shipped freeze-dried
Product Sheet
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Product Sheet