pK (ATCC® 77380)

Applications: expression vectorvector permitting construction of fusion proteinsvector permitting visual detection of recombinants  /  Depositors: JJ McCormick

Permits and Restrictions

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Designations pK
Depositors JJ McCormick
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 2.7660000324249270
Vector: pK (plasmid)
Promoters: Promoter lac
Construction: pUC19
Construct size (kb): 2.766000032424927
Features: insert detection: lacZ'
marker(s): ampR
promoter: lac
replicon: pMB1
expression vector
vector permitting construction of fusion proteins
vector permitting visual detection of recombinants
Restriction digests of the clone give the following sizes (kb): PvuI--1.8, 1.0; EcoRI--2.8; HindIII--2.8; SspI--2.8; ClaI--2.8.
The plasmid contains the following restriction sites (bp from 0): NotI--0; Nsi--75; PvuI--119, 1907; EcoRI--237; HindIII--288; SapI--524; DrdI--749, 2618; BsaI--1607; SspI--2342.
Cloning vector with a second multiple cloning site inserted at the KasI site of pUC19 not interrupting lacZ'.
The order of the major features in this plasmid is: NotI/MCSII/NsiI - 3' lacZ' - EcoRI/MCSI/HindIII - 5' lacZ' - SapI - pMB1 ori - ampR.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Wang Q, et al. Mammalian expression vectors with modulatable promoters and two multiple cloning sites. Gene 119: 155-161, 1992. PubMed: 1327962

J J McCormick, personal communication

Shipped freeze-dried