pNC3 (ATCC® 77217)

Applications: contains sequence protease, neutralexpression vectorvector permitting visual detection of recombinantsvector with sequences for protein export  /  Depositors: L Wang

Permits and Restrictions

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Designations pNC3
Depositors L Wang
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 5.3540000915527340
Vector: pNC3 (plasmid)
Promoters: Promoter nprE
Construction: pWL267, pUC19
Construct size (kb): 5.349999904632568
Features: insert detection: protease
marker(s): kanR
promoter: nprE
replicon: pUB110
contains sequence protease, neutral
expression vector
vector permitting visual detection of recombinants
vector with sequences for protein export
Restriction digests of the clone give the following sizes (kb): BamHI/EcoRI--4.1, 1.15; HindIII--5.2; SalI--5.2; PstI--5.2; EcoRI--5.2.
Expression vector permitting visual detection of recombinants when plated on skim milk indicator plates by the loss or reduction of a halo around a colony.
Contains the signal sequence for the neutral protease, so that recombinants in-frame with the gene may be exported.
Constructed by an in-frame replacement of part of the pro-peptide coding region with part of the MCS from pUC19.
The order of the major features in this plasmid is: nprE (signal peptide coding region - MCS - pro peptide coding region - mature peptide coding region) - ori - kanR.
Media ATCC® Medium 1638: LB medium (ATCC medium 1065) with 5 mcg/ml kanamycin
Growth Conditions
Temperature: 37.0°C

Wu ZR, et al. Development of a novel Bacillus subtilis cloning system employing its neutral protease as screen marker. Gene 106: 103-107, 1991. PubMed: 1937030

Shipped freeze-dried