pCMVADH (ATCC® 77168)

Applications: reporter constructshuttle vector  /  Depositors: LA Culp

Permits and Restrictions

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Designations pCMVADH
Depositors LA Culp
Biosafety Level 1
Vector Information
Size (kb): 9.0000000000000000
Vector: pCMVADH (plasmid)
Promoters: Promoter CMV
Marker(s):neoR
Construct size (kb): 9.0
Features: marker(s): neoR
promoter: CMV, RSV
replicon: pMB1, M13, SV40, polyoma
terminator: SV40
Applications
reporter construct
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--9.2; HindIII/XbaI--7.2, 1.8; SmaI--9.2; StuI--9.2.
Shuttle reporter plasmid permitting visual detection of activity by histochemical staining.
Presence of alcohol dehydrogenase activity is used to follow cell lineage in culture or in situ.
The order of the major features in this plasmid is: CMV promoter - Drosophila ADH-s - SV40 polyadenylation signal - RSV promoter - neoR - polyoma ori - SV40 ori - pMB1 ori.
Media ATCC® Medium 1799: LB medium (ATCC medium 1065) plus 10 mcg/ml neomycin
Growth Conditions
Temperature: 37.0°C
References

Lin WC, Culp LA. Selectable plasmid vectors with alternative and ultrasensitive histochemical marker genes. BioTechniques 11: 344-351, 1991. PubMed: 1931036

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet