pEX11 (ATCC® 37708)

Applications: expression vectorvector for constructing cDNA libraries  /  Depositors: BA Van Der Zeijst

Designations pEX11
Permits and Restrictions

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Depositors BA Van Der Zeijst
Biosafety Level 1
Vector Information
Size (kb): 5.8000001907348630
Vector: pEX11 (plasmid)
Promoters: Promoter lambda PR
Construction: pEX1, oligonucleotide
Construct size (kb): 5.800000190734863
Features: marker(s): ampR
promoter: lambda PR
replicon: pMB1
terminator: phage fd
enhancer: none
expression vector
vector for constructing cDNA libraries
Restriction digests of the clone give the following sizes (kb): EcoRI--5.8; BamHI--5.8; PstI--5.8; EcoRI/EcoRV--4.3, 1.9; SalI--5.8.
This strain is resistant to 100 ug/ml ampicillin.
The recombinant fusion protein generated using this vector is not solubilized in Triton X-100 lysis procedures and is thus easily purified, resistant to proteolysis, and easily detected by direct immunoscreening.
This is one of three bacterial expression vectors (pEX11, ATCC 37708 pEX12, ATCC 37709 and pEX13, ATCC 37710) which differ by translational reading frames and which express cro-lacZ fusion proteins regulated by the PR promoter of bacteriophage lambda.
This was constructed from pEX1 by the ligating a synthetic oligonucleotide into pEX1 cut with XmaI and BamHI. The oligonucleotide added several unique restriction sites.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 30.0°C

Kusters JG, et al. Improvement of the cloning linker of the bacterial expression vector pEX. Nucleic Acids Res. 17: 8007, 1989. PubMed: 2678010

Stanley KK, Luzio JP. Construction of a new family of high efficiency bacterial expression vectors: identification of cDNA clones coding for human liver proteins. EMBO J. 3: 1429-1434, 1984. PubMed: 6086324

Bernard A Van Der Zeijst, personal communication

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