pSV3-neo (ATCC® 37150)

Applications: Expression vector Shuttle vector  /  Depositors: P Berg, Biochemistry Department

Permits and Restrictions

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Designations pSV3-neo
Depositors P Berg, Biochemistry Department
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Intact vector size: 8.60 kb
Type of vector: plasmid
Cloning sites: EcoRI PvuII
Construction: SV40, Tn5, pBR322
Host range: vertebrate cells; Escherichia coli

Features:
marker(s): ampR, G418R
promoter: SV40 early, pBR322
replicon: pMB1

Selectable markers:  ampicillin (E. coli), G418 (mammalian cell lines)
Applications
Expression vector
Shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--8.6; HindIII--4.6, 3.1, 1.4, 0.64; PvuII--4.0, 2.1, 1.1, 0.74, 0.64, 0.37.
Provides dominant selectable marker for resistance to antibiotic G418 in mammalian cell lines.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions

Temperature: 37°C

  

References

Southern PJ, Berg P. Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter. J. Mol. Appl. Genet. 1: 327-341, 1982. PubMed: 6286831

Shipped frozen
Shipping Information Frozen Escherichia coli HB101 containing the plasmid.
Product Sheet
Product Sheet
Product Sheet
Attachments Vector map for 37150