pSV3-neo (ATCC® 37150)

Applications: Expression vector Shuttle vector  /  Depositors: P Berg, Biochemistry Department

Designations pSV3-neo
Permits and Restrictions

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Depositors P Berg, Biochemistry Department
Biosafety Level 1
Vector Information
Intact vector size: 8.60 kb
Type of vector: plasmid
Cloning sites: EcoRI PvuII
Construction: SV40, Tn5, pBR322
Host range: vertebrate cells; Escherichia coli

marker(s): ampR, G418R
promoter: SV40 early, pBR322
replicon: pMB1

Selectable markers:  ampicillin (E. coli), G418 (mammalian cell lines)
Expression vector
Shuttle vector
Restriction digests of the clone give the following sizes (kb): EcoRI--8.6; HindIII--4.6, 3.1, 1.4, 0.64; PvuII--4.0, 2.1, 1.1, 0.74, 0.64, 0.37.
Provides dominant selectable marker for resistance to antibiotic G418 in mammalian cell lines.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions

Temperature: 37°C



Southern PJ, Berg P. Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter. J. Mol. Appl. Genet. 1: 327-341, 1982. PubMed: 6286831

Shipped frozen
Shipping Information Frozen Escherichia coli HB101 containing the plasmid.