Escherichia coli (Migula) Castellani and Chalmers (ATCC® 35695)

Strain Designations: MC4100  /  Type Strain: no  /  Biosafety Level: 1

Permits And Restrictions

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Classification Enterobacteriaceae, Escherichia
Strain Designations MC4100
transformation host
Type Strain no
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Storage Conditions

Conditions: Store unopened frozen vial at -80°C.


Preceptrol® no
Genotype F- araD139 delta(argF-lac)U169 rpsL150 relA1 deoC1 rbsR fthD5301 fruA25 lambda-
Type Strain no
Bacteriophage host
host for lac+ plasmids or lambda phage
Medium ATCC® Medium 1065: LB Agar/Broth, Miller
Growth Conditions
Temperature: 37.0°C
Genotype F- araD139 delta(argF-lac)U169 rpsL150 relA1 deoC1 rbsR fthD5301 fruA25 lambda-
Name of Depositor GM Weinstock
Chain of Custody
ATCC <<--GM Weinstock<<--T. Silhavy <<--- M. Casadaban

Bremer E, et al. Alpha placMu: a transposable derivate of bacteriophage lambda for creating lacZ protein fusions in a single step. J. Bacteriol. 158: 1084-1093, 1984. PubMed: 6327627

Casadaban MJ. Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and mu. J. Mol. Biol. 104: 541-555, 1976. PubMed: 781293

Perkins JD, et al. XbaI and BlnI genomic cleavage maps of Escherichia coli K-12 strain MG1655 and comparative analysis of other strains. J. Mol. Biol. 232: 419-445, 1993. PubMed: 8393935

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation