pGEV-LEU2 plasmid in E. coli (ATCC® MBA-172)

Depositors: J Pinkham

Designations pGEV-LEU2 plasmid in E. coli
Depositors J Pinkham
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 8.6600000000000000
Vector: pGEV-LEU2 plasmid in E. coli (plasmid)
Type: shuttle
Promoters: Promoter for expression MRP7
Construction: pRS315, MRP7, GAL4, Estradiol receptor-alpha, Herpes simplex virus protein 16
A 2.65 kb NotI - BglII fragment containing the MRP7 promoter and the GAL4-hER-VP16 construct was inserted into the NotI and BamHI sites of pRS314. The order of features in the insert are - MRP7 promoter (nt 1-1092) - GAL4 (a.a. 1-93) - Estradiol receptor-alpha (a.a. 282-576) - Herpes Simplex virus protein 16 (a.a. 424-490). The start codon begins at nt 1093. The stop codon begins at nt 2497.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Tightly regulated, B-Estradiol Dose-Dependent Expression System for Yeast. BioTechniques 29: 1226-1231, 2000.

Shipped frozen
Shipping Information Frozen glycerol stock of Escherichia coli GC10 containing the plasmid.