ASSg3 (ATCC® 59880)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: H Northrup, WE O'Brien

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Designations ASSg3
Species Homo sapiens, human
Depositors H Northrup, WE O'Brien
Construct size (kb): 8.800000190734863
DNA: genomic
Insert lengths(kb): 4.400000095367432
Tissue: fetal liver
Gene product: argininosuccinate synthetase(arginosuccinate synthetase 1) [ASS]
Alleles: A1, A1, A1, A2, A2, A2
Insert Size (kb) 4.400
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
When excised from pBR322 with EcoRI + HindIII, insert and vector are about the same size.
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--4.4; EcoRI--10.0; BamHI--10.0; BamHI/EcoRI--5.0, 4.0; PvuII--4.4.
Insert contains exons 11 and 12 as well as some intron sequence of the argininosuccinate synthetase gene.
The population studied was CEPH (Centre d'Etude du Polymorphisme Humain) families.
Enzyme(s) not detecting polymorphism: EcoRV, KpnI, ScaI, SphI, SstI, BanI, BclI, BglI, BglII, EcoRI, HincII, BanII, Fnu4HI, MboI, RsaI, TaqI, HindIII, PssI, PvuII, StuI, XbaI.

Northrup H, et al. Multilocus linkage analysis with the human argininosuccinate synthetase gene. Genomics 5: 442-444, 1989. PubMed: 2575581

Hope Northrup, personal communication