CCD 1108Sk (ATCC® CRL-2352)

Organism: Homo sapiens, human  /  Cell Type: fibroblast  /  Disease: Lymphoma

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Organism Homo sapiens, human
Cell Type fibroblast
Product Format frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 1
Disease Lymphoma
Age 24 years
Gender male
Ethnicity Caucasian, White
Applications
The line was established from skin taken from the leg (below the knee amputation) of a 24 year old White male with malignant lymphoma.
The cells have been observed to senesce at about 32 population doublings.
Derivation
The line was established from skin taken from the leg (below the knee amputation) of a 24 year old White male with malignant lymphoma.
Clinical Data
The line was established from skin taken from the leg (below the knee amputation) of a 24 year old White male with malignant lymphoma.
male
Caucasian, White
24 years
Comments
The line was established from skin taken from the leg (below the knee amputation) of a 24 year old White male with malignant lymphoma.
Cells from the current passage (1) have experienced 3 population doublings beyond the biopsy material.
The cells have been observed to senesce at about 32 population doublings.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Cryopreservation
growth medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Name of Depositor A Thompson
Passage History
Cells from the current passage (1) have experienced 3 population doublings beyond the biopsy material.
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation