NBT-II [Nara Bladder Tumor No. 2] (ATCC® CRL-1655)

Organism: Rattus norvegicus, rat  /  Tissue: urinary bladder  /  Disease: tumor

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Organism Rattus norvegicus, rat
Tissue urinary bladder
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease tumor
Gender male
Strain Wistar
Storage Conditions liquid nitrogen vapor phase
Derivation
The line was derived from a tumor which was induced with N-butyl-N-(4-hydroxybutyl) nitrosamine.
Comments NBT-II cells differentiate in vitro giving rise to tumor giant cells.

The differentiation is inhibited by vitamin A.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.03% (w/v) EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.  Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. 
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: 2 to 3 times per week
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994
Cryopreservation
Complete growth medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
Name of Depositor J Leighton, R Tchao
References

Toyoshima K, et al. Tissue culture of urinary bladder tumor induced in a rat by N-butyl-N-(- 4-hydroxybutyl)nitrosamine: establishment of cell line, Nara Bladder Tumor II. J. Natl. Cancer Inst. 47: 979-985, 1971. PubMed: 4941696

Geisinger KR, et al. Appearance of giant cells as a morphological response of rat bladder carcinoma cell line (Nara Bladder Tumor No. 2) to a continuous thermal gradient in tissue culture. Cancer Res. 38: 1223-1230, 1978. PubMed: 639055

Toyoshima K, Leighton J. Vitamin A inhibition of keratinization in rat urinary bladder cancer cell line Nara Bladder Tumor No. 2 in meniscus gradient culture. Cancer Res. 35: 1873-1879, 1975. PubMed: 1093679

Tchao R. Novel forms of epithelial cell motility on collagen and on glass surfaces. Cell Motil. 2: 333-341, 1982. PubMed: 7151140

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Toyoshima K, et al. Tissue culture of urinary bladder tumor induced in a rat by N-butyl-N-(- 4-hydroxybutyl)nitrosamine: establishment of cell line, Nara Bladder Tumor II. J. Natl. Cancer Inst. 47: 979-985, 1971. PubMed: 4941696

Geisinger KR, et al. Appearance of giant cells as a morphological response of rat bladder carcinoma cell line (Nara Bladder Tumor No. 2) to a continuous thermal gradient in tissue culture. Cancer Res. 38: 1223-1230, 1978. PubMed: 639055

Toyoshima K, Leighton J. Vitamin A inhibition of keratinization in rat urinary bladder cancer cell line Nara Bladder Tumor No. 2 in meniscus gradient culture. Cancer Res. 35: 1873-1879, 1975. PubMed: 1093679

Tchao R. Novel forms of epithelial cell motility on collagen and on glass surfaces. Cell Motil. 2: 333-341, 1982. PubMed: 7151140