Karyotype
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modal number = 80; range = 68 to 83. This is a hypertriploid human cell line with the modal chromosome number of 80 occurring in 44% of a total of 88 cells examined. The rate of polyploid cells was 3.5%. Karyotypes of this cell line were generally uniform and stable. There were 12-13 marker chromosomes per cell, 11 of which were found in all cells, and 7 of which were mostly paired. Among the markers were paired mar(1qter--q21::?::6p11.2--6pter), t(3q::?), and i(16q); single der(12)t(12;?)(q24:?); and 8-9 others. Double minutes occurred in some cells; however, they were present as only one or two copies per cell. Structurally normal N16 was absent and N8 occurred in only a few cells. Paired normal X chromosomes were present in every cell.
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Derivation
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The CAMA-1 line was established by J. Fogh at Sloan-Kettering in 1975 from cells in the pleural effusion of a patient with carcinoma of the breast.
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Clinical Data
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51 years
Caucasian
female
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Antigen Expression
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Blood type O; Rh +; HLA A10, A11, B12, B18
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Comments
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An ampule frozen in May of 1978 at passage 21 was provided to the ATCC in June of 1991.
The cells form poorly differentiated grade III tumors consistent with breast carcinoma.
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