Human adenovirus 3 (ATCC® VR-3)

Classification: Adenoviridae, Mastadenovirus, Human adenovirus B  /  Product Format: frozen 1 mL per vial

Permits and Restrictions

View Permits

Classification Adenoviridae, Mastadenovirus, Human adenovirus B
Deposited As Adenovirus type 3
Agent Human adenovirus 3
Strain GB
Applications
Respiratory research
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Comments Agglutinates rhesus monkey red blood cells. Weakly oncogenic, contains soluble group-specific CF antigen.
Effect on Host
CPE, rounding, clumping, and sloughing
Recommended Host
A549 (ATCC® CCL-185™)
Growth Conditions
Temperature: 37°C
Recommendations For Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 70-85% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 2-3 days
Effect on Host
CPE, rounding, clumping, and sloughing
Name of Depositor RJ Huebner, National Institute of Allergy and Infectious Diseases
Source Nasal washings from an adult male with a common cold, Maryland, 1953
References

Hierholzer JC, et al. Antigenic relationships among the 47 human adenoviruses determined in reference horse antisera. Arch. Virol. 121: 179-197, 1991. PubMed: 1759904

Ma Y, Mathews MB. Structure, function, and evolution of adenovirus-associated RNA: a phylogenetic approach. J. Virol. 70: 5083-5099, 1996. PubMed: 8764016

Pereira HG, et al. A short description of the adenovirus group. Virology. 20: 613-620, 1963. PubMed: 14059828

Rowe WP, et al. Studies of the adenoidal-pharyngeal-conjunctival (APC) group of viruses. Am. J. Hyg. 61: 197-218, 1955.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation