Zika virus (ATCC® VR-1838)

Classification: Flaviviridae, Flavivirus  /  Product Format: frozen 1.0 mL

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Classification Flaviviridae, Flavivirus
Deposited As Zika
Agent Zika virus
Strain MR 766 (Tissue culture-adapted from ATCC® VR-84™)
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 1.0 mL
Storage Conditions -70°C or colder
Comments Derivation by adaptation from the in vivo cultured product ATCC® VR-84™ to Vero cells (ATCC® CCL-81™). The genomic sequences of ATCC® VR-84™ and ATCC® VR-1838™ have not been compared, and ATCC® VR-1838™ has not been tested for infectivity in vivo.
Effect on Host
CPE, Refractile cell rounding
Recommended Host
Vero cells (ATCC® CCL-81™)
Growth Conditions

Temperature: 37°C

Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 75-80% confluent. Remove medium and then wash monolayer with PBS or serum free medium prior to inoculation. Inoculate with a small volume of inoculum (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1-0.01). Adsorb for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.

Incubation: 2-4 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80-90% of the monolayer.

Effect on Host
CPE, Refractile cell rounding
Name of Depositor J Casals, The Rockefeller Foundation; ATCC
Source Blood of a rhesus monkey which became infected while stationed as a sentinel in forest near Entebbe, Uganda, 1947
Year of Origin 1947
References

Dick GW, Kitchen SF, and Haddow AJ. Zika Virus. I. Isolations and Serological Specificity. Trans R Soc Trop Med Hyg 46: 509-520, 1952. PubMed: 12995440

Zhu Z, et al. Comparative genomic analysis of pre-epidemic and epidemic Zika virus strains for virological factors potentially associated with the rapidly expanding epidemic. Emerg Microbes Infect 5: e22, 2016.

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