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Influenza A virus (H1N1) (ATCC® VR-1736™)

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Classification: Orthomyxoviridae, Influenzavirus A  /  Product Format: frozen

Permits and Restrictions

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Classification Orthomyxoviridae, Influenzavirus A
Agent Influenza A virus (H1N1)
Strain A/Virginia/ATCC1/2009
Verified By Whole-genome Sequencing
Applications
Emerging infectious disease research
Respiratory research
Influenza Research
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format frozen
Storage Conditions -70°C or colder
Comments
Item analyzed using Real-time RT-PCR to determine strain is of pandemic swine Influenza A origin.
Effect on Host
CPE, rounded refractile cells with cell degeneration
Recommended Host
MDCK (ATCC® CCL-34™™)
Growth Conditions

Recommendations for Infection:  Plate cells 24 hours in advance and infect when cultures are 80-90% confluent.   Remove medium and inoculate with a small volume of virus diluted with VGM to provide a MOI 0.1. Adsorb for 1 hour at 35°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.

Incubation: Incubate infected culture for 2 to 5 days at 35°C in a humidified 5% CO2 atmosphere, until CPE are well advanced through 90% of the culture.
Effect on Host
CPE, rounded refractile cells with cell degeneration
Name of Depositor ATCC®
Special Collection ATCC
Source Nasopharyngeal specimen from a patient positive for Flu A in Virginia, 2009.
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
SDS
SDS
FAQs
  1. Accessing genome sequencing data


    Date Updated: 9/30/2019

  2. Criteria for reference quality genomes


    Date Updated: 9/30/2019