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Betacoronavirus 1 (ATCC® VR-1558)

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Classification: Coronaviridae, Betacoronavirus  /  Product Format: frozen 1 mL per vial

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Classification Coronaviridae, Betacoronavirus
Deposited As Human coronavirus OC43
Agent Betacoronavirus 1
Strain OC43
Common Name Human coronavirus OC43
Verified By Whole-genome Sequencing
Applications
Respiratory research
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Images Image of Host Effects for VR-1558
Comments VR-1558™ is a TC-adapted item derived from virus passaged in mice (ATCC® VR-759™).  Cleaned of mycoplasma with MRA.  No antigenic cross reaction with Human coronavirus 229E (ATCC® VR-740™).

Next-generation sequencing (NGS) at ATCC on the HCT-8 [HRT-18] cell line (ATCC® CCL-244™) used as the host has shown the presence of a putative fragment of Gammaherpesvirus with a ≥98% homology to Human Heparanase 2 (NCBI number NG_023416.1).
Effect on Host
CPE, vacuolization of cytoplasm and sloughing
Recommended Host
HCT-8 (ATCC® CCL-244™)
Growth Conditions Temperature: 33°C
Recommendations for Infection: For best results, infection should be performed on an 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2), optimized for propagation (e.g. MOI 0.01-0.1 recommended). Wash monolayer two times with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 33°C in a humidified 5% CO2 atmosphere, while rocking continuously. End adsorption by adding virus growth medium.
Incubation: 4-6 days at 33°C in a humidified 5% CO2 atmosphere, rocking continuously, until CPE is progressed through 80% of the monolayer.
Effect on Host
CPE, vacuolization of cytoplasm and sloughing
Name of Depositor JO Fleming, University of Wisconsin, Madison
Special Collection ATCC
Chain of Custody ATCC <-- JO Fleming <-- MK Cooney <-- AZ Kapikian
Source Man with cold-like illness. Treated to remove mycoplasma at ATCC.
References

Bruckova M, et al. The adaptation of two human coronavirus strains (OC38 and OC43) to growth in cell monolayers. Proc. Soc. Exp. Biol. Med. 135: 431-435, 1970. PubMed: 4991398

McIntosh K, et al. Growth in suckling-mouse brain of "IBV-like" viruses from patients with upper respiratory tract disease. Proc. Natl. Acad. Sci. USA 58: 2268-2273, 1967. PubMed: 4298953

McIntosh K, et al. Recovery in tracheal organ cultures of novel viruses from patients with respiratory disease. Proc. Natl. Acad. Sci. USA 57: 933-940, 1967. PubMed: 5231356

Cross References

Nucleotide (GenBank) : NC_005147.1 Human coronavirus OC43, complete genome

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
FAQs
  1. Accessing genome sequencing data


    Date Updated: 9/30/2019

  2. Criteria for reference quality genomes


    Date Updated: 9/30/2019