|
Depositors
|
R Weiss, Massachusetts Institute of Technology
|
|
Applications
|
An entry vector carrying a weak constitutive promoter for gene expression in Saccharomyces cerevisiae
|
|
Vector
|
Total size of construct (kb): 3.425
Vector type: Entry vector
Markers: kanR
|
|
Insert
|
Insert size (kb): 0.73
Suggested primers for sequencing:
M13 Forward: 5’- GTTTTCCCAGTCACGAC - 3’
M13 Reverse: 5’- CAGGAAACAGCTATGAC - 3’
|
|
Preparation
|
Before opening the vial, centrifuge at 6,000 x g for 30 seconds. Add 30 µL of Molecular Grade Water and incubate the vial at 4°C overnight to dissolve the DNA. Each vial contains 2-3 µg plasmid DNA (measured by PicoGreen® dsDNA quantitation assay).
|
|
Related Products
|
ATCC SB-1008
ATCC SB-1035
ATCC SB-1036
ATCC SB-1041
ATCC SB-1044
ATCC SB-1104
ATCC SB-1105
|
|
Biosafety Level
|
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
|
|
References
|
Chen MT, Weiss R. Artificial cell-cell communication in yeast Saccharomyces cerevisiae using signaling elements from Arabidopsis thaliana. Nat Biotechnol 23(12): 1551–1555, 2005. PubMed: 16299520
|