SCC-9 (ATCC® CRL-1629)

Tissue: tongue  /  Disease: squamous cell carcinoma

Permits and Restrictions

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Tissue tongue
Product Format frozen
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease squamous cell carcinoma
Age 25 years
Gender male
Applications This cell line is a suitable transfection host.
Clinical Data
25 years
Genes Expressed
epidermal keratins; low levels of involucrin
Cellular Products
epidermal keratins; low levels of involucrin
Tumorigenic Yes
Yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10cells.
Growth of SCC-9 is enhanced by using a feeder layer of Mitomycin C-treated STO cells (ATCC 56-X.2).
The cells do not grow well in semi-solid medium.
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 11
D13S317: 9
D16S539: 10,11
D5S818: 12
D7S820: 8
THO1: 8,9
TPOX: 9,11
vWA: 17
Name of Depositor JG Rheinwald
Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation