Arcanobacterium haemolyticum (MacLean et al.) Collins et al.

1. Open vial according to enclosed instructions or visit www.atcc.org for instructions.
2. Rehydrate the entire pellet with approximately 0.5 mL of #4 broth. Aseptically transfer the entire contents to a 5-6 mL tube of #4 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary broth tubes.
3. Use several drops of the primary broth tube to inoculate a #4 plate and/or #4 agar slant.
4. Incubate at 37°C for 1-3 days in an atmosphere of 5% CO₂.

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