Distribution host: Escherichia coli HB101 (ATCC 33694)
Size (kb): 4.7109999656677250
DESCRIPTION OF VECTOR:
Intact vector size: 4.711
Type of vector: plasmid
Cloning sites: HindIII SalI BamHI ClaI SmaI KpnI SacI
Polylinker sites: HindIII SalI BamHI [tk-promoter luc+ SV40 poly (A)] ClaI
SmaI KpnI SacI
Other unique sites: NarI BclI FseI ClaI SmaI KpnI SacI PvuII BsaI BglI FspI PvuI
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
MCS: HindIII...BamHI, ->, 1-36
promoter: TK, 37-200
reporter group: luciferase (luc+), ->, 238-1890
terminator: SV40 late polyadenylation, 1922-2143
MCS: ClaI...SacI, 2146-2168
replicon: pMB1, 2587-2587
marker(s): ampR, <-, 3345-4205
terminator: SV40 large T-antigen polyadenylation, 4337-4708
Vector: ptkLUC+ (plasmid)
Promoters: Promoter TK
Construct size (kb): 4.710999965667725
Features: marker(s): ampR
reporter group: luciferase (luc+)
terminator: SV40 large T-antigen polyadenylation
terminator: SV40 late polyadenylation
Restriction digests of the clone give the following sizes (kb): BamHI--4.7; HindIII--4.7.
The vector contains modified luciferase (luc+) gene from the firefly Photinus pyralis in order to increase the yield of recoverable luciferase activity after transfection and to eliminate potential cryptic regulatory elements.
The promoters driving luc+ transcription in these two vectors represent different 5' deletions of the tk promoter ending at -32 and -105, respectively.
The optimized luciferase reporter gene vectors (ATCC 87630 - 87633) provide valuable tools for the analysis of eukaryotic regulatory DNA elements.
The vectors pTATALUC+ (ATCC 87631)
and ptkLUC+ (ATCC 87632)
were designed for the characterization of isolated cis regulatory elements.