M3499 [URA3 --> ADE2 converter] (ATCC® 87558)

Applications: YI-type (integrating) shuttle vectorhost modificationmarker swap vector URA3 --> ADE2  /  Depositors: DJ Stillman

Permits and Restrictions

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Designations M3499 [URA3 --> ADE2 converter]
Depositors DJ Stillman
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 7.4260001182556150
Vector: M3499 (plasmid)
Construction: pUC18
Marker(s):ampR ADE2
Construct size (kb): 7.426000118255615
Features: gene disruption cassette: ura3::ADE2
marker(s): ampR
replicon: pMB1
restriction site: HindIII
restriction site: HindIII...EcoRI
restriction site: PstI
Applications
YI-type (integrating) shuttle vector
host modification
marker swap vector URA3 --> ADE2
Comments
Restriction digests of the clone give the following sizes (kb): HindIII--2.5, 1.3, 1.1, 1.1, 1.0; BamHI--6.6, 0.5; PvuI--6.2, 0.8.
To convert the host phenotype from URA3 to ADE2, transform with the PstI digested vector (4.45 kb) and select for Ade+ transformants.
A marker swap (URA3->ADE2) vector for altering a Saccharomyces cerevisiae host by a one-step gene disruption.
When swapping markers on an episomal plasmid, appropriate phenotype may result from loss of the plasmid unless a second selectable or scorable marker is used to ensure plasmid maintenance.
Vector was constructed by replacing an internal StuI-EcoRV fragment of URA3 with a 3.7 kb BamHI fragment containing the ADE2 coding sequence. ADE2 and URA3 are in the same orientation.
Growth Conditions
Temperature: 37.0°C
References

Kasten MM, et al. A large protein complex containing the yeast Sin3p and Rpd3p transcriptional regulators.. Mol. Cell. Biol. 17(8): 4852-4858, 1997. PubMed: 9234741

Related Products
component of:ATCC 87561
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Product Sheet
Product Sheet
Product Sheet
Attachments Vector Map for 87588