YCp50-poly (ATCC® 87555)

Applications: YC-type (centromeric) shuttle vectorvector for constructing genomic libraries  /  Depositors: DJ Stillman

Permits and Restrictions

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Designations YCp50-poly
Depositors DJ Stillman
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 7.0560002326965330
Vector: YCp50-poly (plasmid)
Construction: YCp50, pUC19 polylinker
Construct size (kb): 7.056000232696533
Features: marker(s): URA3
marker(s): ampR
replicon: ARS1
replicon: pMB1
MCS: EcoRI...HindIII
centromere: CEN4
YC-type (centromeric) shuttle vector
vector for constructing genomic libraries
Restriction digests of the clone give the following sizes (kb): EcoRI--7.2; SmaI--5.1, 2.2; PstI--3.3, 1.8, 1.1, 0.9; PvuI--7.2.
YC-type shuttle vector.
YCp50-poly was constructed in two steps: (1) YCp50 (ATCC 37419) was cleaved with XhoI and Asp718, blunted and ligated. (2) This construct was then cleaved with EcoRI and SalI, and the EcoRI-PvuII fragment of pUC19 was inserted.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Rose MD, et al. A Saccharomyces cerevisiae genomic plasmid bank based on a centromere-containing shuttle vector. Gene 60: 237-243, 1987. PubMed: 3327750

Wang H, Stillman DJ. Transcriptional repression in Saccharomyces cerevisiae by a SIN3-lexA fusion protein. Mol. Cell. Biol. 13: 1805-1814, 1993. PubMed: 8441414

Related Products
component of:ATCC 87635
Shipped freeze-dried
Shipping Information

 Freeze dried E. coli containing the plasmid.