Size (kb): 6.7709999084472660
Vector: YEpFAT10 (plasmid)
Promoters: Promoter for in vitro transcription T7
Construct size (kb): 6.770999908447266
Features: marker(s): LEU2-d
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: 2 micron ori/LEU2-d
YE-type (episomal) shuttle vector
Restriction digests of the clone give the following sizes (kb): EcoRI-- 4.2, 2.9; BamHI-- 7.0; SalI-- 7.0.
Selection for TRP1 expression produces a plasmid copy number of 20-40; growth on YC minus leucine produces a copy number of 100-200.
Similarly, the NruI, BamHI, SalI and SphI are adjacent and can be used for directional cloning. The vector was constructed by Kurt Runge.
One of 9 YE-type shuttle vectors (ATCC 87541
, 87554, 87560 an d 87640 - 87645) that have the leu2-d gene, which is correlated with high copy number in S. cerevisiae, when grown in medium lacking leucine.
Conrad MN, et al. RAP1 protein interacts with yeast telomeres in vivo: overproduction alters telomere structure and decreases chromosome stability. Cell 63: 739-750, 1990. PubMed: 2225074
Runge KW, Zakian VA. Introduction of extra telomeric DNA sequences into Saccharomyces cerevisiae results in telomere elongation. Mol. Cell. Biol. 9: 1488-1497, 1989. PubMed: 2657397
Freeze dried stock of Escherichia coli containing the plasmid.