YEpFAT10 (ATCC® 87554)

Applications: YE-type (episomal) shuttle vector  /  Depositors: DJ Stillman, K Runge

Permits and Restrictions

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Designations YEpFAT10
Depositors DJ Stillman, K Runge
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 6.7709999084472660
Vector: YEpFAT10 (plasmid)
Promoters: Promoter for in vitro transcription T7
Construction: pVZ1
Construct size (kb): 6.770999908447266
Features: marker(s): LEU2-d
marker(s): TRP1
marker(s): ampR
promoter: lac
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: 2 micron ori/LEU2-d
replicon: pMB1
MCS: ApaI...SacII
MCS: NruI...SphI
YE-type (episomal) shuttle vector
Restriction digests of the clone give the following sizes (kb): EcoRI-- 4.2, 2.9; BamHI-- 7.0; SalI-- 7.0.
Selection for TRP1 expression produces a plasmid copy number of 20-40; growth on YC minus leucine produces a copy number of 100-200.
Similarly, the NruI, BamHI, SalI and SphI are adjacent and can be used for directional cloning. The vector was constructed by Kurt Runge.
One of 9 YE-type shuttle vectors (ATCC 87541, 87554, 87560 an d 87640 - 87645) that have the leu2-d gene, which is correlated with high copy number in S. cerevisiae, when grown in medium lacking leucine.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Conrad MN, et al. RAP1 protein interacts with yeast telomeres in vivo: overproduction alters telomere structure and decreases chromosome stability. Cell 63: 739-750, 1990. PubMed: 2225074

Runge KW, Zakian VA. Introduction of extra telomeric DNA sequences into Saccharomyces cerevisiae results in telomere elongation. Mol. Cell. Biol. 9: 1488-1497, 1989. PubMed: 2657397

Shipped freeze-dried
Shipping Information

 Freeze dried stock of Escherichia coli containing the plasmid.

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