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Host
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Distribution host: Escherichia coli DH5alpha
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Vector Information
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Size (kb): 4.3839998245239260 DESCRIPTION OF VECTOR:
Intact vector size: 4.384
Type of vector: phagemid
Cloning sites: KpnI XhoI SalI ClaI HindIII EcoRI SmaI BamHI SpeI XbaI EagI
NotI BstXI SacII SacI NaeI
Polylinker sites: BssHII KpnI ApaI XhoI SalI ClaI HindIII EcoRV EcoRI PstI
SmaI BamHI SpeI XbaI EagI NotI BstXI SacII SacI BssHII
Construction: pBluescriptII KS+
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
marker(s): URA3, ->, 417-1219
replicon: f1, <-, 1351-1807
promoter for in vitro transcription: T7, ->, 1977-1996
MCS: KpnI...SacI, ->, 2004-2111
promoter for in vitro transcription: T3, <-, 2123-2142
insert detection: lacZ', <-, 1808-2167
promoter: lac, <-, 2212-2240
replicon: pMB1, 2566-2566
marker(s): ampR, <-, 3324-4184 Vector: pRS406 (phagemid) Promoters: Promoter for in vitro transcription T7 Construction: pBluescriptII KS+ Marker(s):URA3,ampR Construct size (kb): 4.383999824523926 Features: insert detection: lacZ'
marker(s): URA3
marker(s): ampR
promoter: lac
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7
replicon: f1
replicon: pMB1
MCS: KpnI...SacI
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Applications
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YI-type (integrating) shuttle vector
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Comments
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Restriction digests of the clone give the following sizes (kb): PvuII--3.95, 0.45; BglI--2.7, 1.7; PstI--2.75, 1.65. A YI-type shuttle vector with URA3 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter system for visual detection of recombinants, promoters for transcription in vitro, and an extensive polylinker flanked by BssHII sites.
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References
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Sikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27, 1989. PubMed: 2659436
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Related Products
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Shipping Information
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Distributed: freeze-dried
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